灵芝漆酶催化活性艳色蓝X-BR脱色条件的研究  

Study on the Decolorized Condition on Reactive Brilliant Blue X-BR by Laccase of Ganoderma lucidum

作　　者：韩业昊 张超[1] 凌庆枝[2] 

机构地区：[1]安徽理工大学医学院,安徽淮南232001 [2]浙江医药高等专科学校,浙江宁波315100

出　　处：《安徽农业科学》2014年第26期8875-8877,共3页Journal of Anhui Agricultural Sciences

基　　金：浙江省科技厅公益技术研究社会发展项目(2010C33072)

摘　　要：[目的]研究灵芝(Ganoderma lucidum)固体发酵所得漆酶粗酶液对蒽醌染料进行脱色的条件。[方法]分别研究不同的pH、染料浓度、温度、酶用量、反应时间等条件对活性艳色蓝X-BR染料脱色的影响。[结果]脱色的适宜条件为:在pH 3.5、40℃、60 U/ml灵芝漆酶、200 mg/L染料浓度的条件下反应5 h,活性艳色蓝X-BR染料脱色率可达49%。[结论]灵芝固体发酵的漆酶粗酶液在一定条件下对蒽醌染料活性艳色蓝X-BR具有较好的脱色效果。[ Objective ] Decolorization of anthraquinone dye with laccase from C, anoderma lucidum was studied. [ Method] Effects of different pH, dye concentrations, temperature, enzyme dosage, reaction time on decolorization of Reactive Brilliant Blue X-BR were studied. [ Result ] The optimal conditions of decolorization are： pH 3.5,40 ℃, 60 U/ml Ganoderma laccase and 200 mg/L dye concentration, respectively. Un- der the above conditions, the decolorization rate of Reactive Brilliant Blue X-BR was 49% after 5 h catalyzation with the laccase from G. luci- dum. [Conclusion] Ganoderma laccase under optimal conditions for Reactive Brilliant Blue X-BR has a decolorization effect.

关 键 词：灵芝漆酶 脱色 

分 类 号：S188[农业科学—农业基础科学]