微波前处理对水稻秸秆酶解性能的影响  

Effect of Microwave Pretreatment on Enzymatic Saccharification of Rice Straw

作　　者：沈洁[1] 陆筑凤[1] 于建兴[1] 叶小梅 李加友[1] 常志洲 

机构地区：[1]嘉兴学院生物与化学工程学院,浙江嘉兴314001 [2]江苏农业科学研究院农业资源与环境研究所,江苏南京210014

出　　处：《安徽农业科学》2014年第26期9088-9090,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省自主创新项目[CX(12)1002]

摘　　要：[目的]利用微波预处理破坏秸秆中半纤维素、纤维素和木质素复合物,改善秸秆质地和结构,提高其生物降解效率,为秸秆生物质能开发创造条件。[方法]通过Box-Behnken设计和响应面法对水稻秸秆进行微波预处理优化试验,并通过秸秆结构表征比对来考察微波处理对水稻秸秆酶解性能的影响。[结果]利用响应面法得到微波前处理的最佳操作参数:功率(MI)700 W,反应时间(IT)28min,固液比(SC)75 g/L。在最优条件下,秸秆的还原糖收率为11.86%,比未处理时增长了47.33%,与秸秆结构的FTIR、电镜等表征结果相符。[结论]微波预处理会破坏秸秆表面的蜡质和硅化细胞,还可以部分分解木质素与半纤维素复合物,提高了水稻秸秆的生物降解性能,具有良好的应用前景。[ Objective ] Microwave pretreatment was used to disrupt the lignin -hemicellulose complex and enhance enzymatic saccharification of rice straw. [ Method] In this study, Box - Behnken design and response surface methodology were employed to plan experiments and optimize the microwave pretreatment of rice straw. [ Result ] According to regression equation to determine, the best microwave pretreatment conditions were as follow： microwave intensity （MI） 700 W, irradiation time （IT） 28 min and substrate concentration （SC） 75 g/L. Under the optimal condi- tions, the maximal recover of reducing sugar was 11.86%, increased by 47.33% compared with untreated straw. The physicochemical surface characteristics analysis of straw further confirmed the perspective. [ Conclusion] Microwave pretreatment could disrupt the silicified waxy surface, break down the lignin-hemicellulose complex and partially remove silicon and lignin, largely enhance enzymatic saccharification of rice straw.

关 键 词：微波前处理 水稻秸秆 响应面分析 还原糖收率 

分 类 号：S181.3[农业科学—农业基础科学] X703.5[环境科学与工程—环境工程]