环孢素A改善创伤失血性休克大鼠血管及器官功能  

Cyclosporin A improves the vascular and organ function after traumatic hemorrhagic shock

作　　者：雷艳[1,2] 董兆君[2] 

机构地区：[1]第三军医大学卫勤训练基地医学综合教研室,重庆400038 [2]第三军医大学军事预防医学院毒理学研究所,重庆400038

出　　处：《基础医学与临床》2014年第9期1171-1174,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(81270400)

摘　　要：目的观察环孢素A(Cs A)对创伤失血性休克大鼠血管收缩/舒张功能、重要器官血流量和功能及线粒体功能的影响,以探讨Cs A发挥抗休克作用的途径。方法采用大鼠创伤失血性休克模型(维持血压40 mm Hg 3 h,伴左侧股骨折断),分为6组:对照组、休克组、乳酸林格氏液(LR)组、Cs A 1、5和10 mg/kg组,每组n=8。在休克模型建立后,按照分组分别输注LR或Cs A,然后用离体血管张力测定技术观察肠系膜上动脉(SMA)血管收缩和舒张功能,用激光多普勒血流监测仪检测肝/肾血流量,用生化分析法测定肝肾功能,用线粒体呼吸控制率检测法测定肝、肾、肠的线粒体功能。结果休克损伤使大鼠SMA的收缩反应性和舒张反应性都明显降低,休克组血管最大收缩反应和最大舒张率分别为正常组的59.3%和71.8%(P<0.01),Cs A可明显恢复休克大鼠的血管收缩/舒张功能,其中Cs A 5 mg/kg组的最大收缩反应性和最大舒张率分别恢复至正常组的93.4%和91.1%,显著高于LR组(P<0.01,P<0.05)。同时,Cs A 5 mg/kg也明显提高了休克动物肝/肾组织血流量,改善肝肾功能和肝、肾、肠的线粒体功能(P<0.01,P<0.05)。结论 Cs A可恢复休克后血管功能,提高器官血流量,改善器官和线粒体功能,发挥对创伤休克的治疗作用。Objective To observe the effect of cyclosporin A （CsA） on the vasoconstriction/vasodilation of the su- perior mesenteric artery （SMA）, vital organ and mitochondrial function after traumatic hemorrhagic shock in rats. Methods With the traumatic hemorrhagic shock model of rats （blood pressure maintained at 40 mmHg and femur was fractured）, rats were divided into six groups： sham-operated, shock, LR, CsA 1, 5, 10 mg/kg （n = 8/ group）. After shock, rats received an infusion of LR or CsA, then the vascular constriction/relaxation of SMA was observe with isolated organ perfusion system, the blood flow in liver and kidney was measured by a laser doppler blood flowmeter, the function of the liver and kidney was measured using biochemical analysis, and the mitochon- drial function of the liver, kidney and small intestine was examined by the respiration control rate method. Results After shock, both the vascular constriction reactivity and the vasodilator reactivity were significantly decreased.CsA markedly restored the decreased constriction and relaxation of SMA after ＂shock （ P 〈 0.01, P 〈 0.05 ）, 5 mg/kg of CsA showed a better effect. CsA （5 mg/kg） also improved tissue bloodflow, organ and mitochondrial function in shock rats. Conclusions CsA has positive effects on traumatic hemorrhagic shock through improving vascular function, increasing tissue blood flow, protecting organ and mitochondrial function.

关 键 词：创伤 失血性休克 器官功能 

分 类 号：R965[医药卫生—药理学]