骨髓间充质干细胞对阿霉素肾病大鼠骨代谢的影响  

Effects of bone marrow mesenchymal stem cells on the bone metabolism of adriamycin induced nephritic rats

作　　者：林秀芹[1] 郑京[2] 刘加林[3] 刘慈赟[1] 吴心虹[1] 张娟[2] 陈小英[1] 

机构地区：[1]福建中医药大学,福建福州350001 [2]福建中医药大学附属人民医院肾内科,福建福州350004 [3]贵州省人民医院肾内科,贵州贵阳550000

出　　处：《基础医学与临床》2014年第9期1215-1220,共6页Basic and Clinical Medicine

基　　金：福建省自然科学基金(2012J01378)

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)对阿霉素肾病大鼠骨代谢的影响。方法制作大鼠阿霉素肾病模型,将50只SD大鼠随机分为5组,即A组:正常组,B组:模型组,C组:激素组,D组:干细胞组,E组:干细胞+激素组。各组于造模后7、21和35 d收集24 h尿标本,以双缩脲比色法测定大鼠尿蛋白,以ELISA法测定血清骨保护蛋白(OPG)、核因子κB受体活化因子配体(RANKL)的水平,用实时定量PCR和Western blot检测胫骨组织中OPG和RANKL的表达。结果与A组比较,第7天,各组尿蛋白升高(P<0.05);与B组比,第21天和35天,C组、D组、E组尿蛋白降低(P<0.05)。与B组比较,C组血清RANKL增多,OPG减少(P<0.05);与C组比较,D组和E组血清OPG表达增多(P<0.05,P<0.01),RANKL减少(P<0.05);与D组比较,E组血清OPG减少、RANKL增多(P<0.05)。与B组比较,C组骨组织OPG蛋白及mRNA减少(P<0.05,P<0.01),RANKL蛋白及mRNA增多(P<0.01);与C组比较,D组和E组骨组织OPG蛋白及mRNA增多(P<0.05),RANKL蛋白及mRNA的表达减少(P<0.01)。与D组比较,E组骨组织OPG蛋白及mRNA减少、RANKL蛋白增多(P<0.05,P<0.01)。结论BMSCs可能通过OPG/RANKL/RANK通路促进成骨细胞分化,抑制破骨细胞生成,从而减缓泼尼松诱导的骨质疏松的形成。Objective To investigate the effects of bone marrow mesenchymal stem cells （BMSCs） on the bone metabolism in adriamycin-induced nephritic rats. Methods Generate adriamycin-induced nephritic rats （n = 50 ）, and randomly divide them into five groups as group A： the normal group, group B： the model group, group C： the hormone group, group D： nephritic rats with stem cells transplant, group E：nephritic rats with stem cells transplant ＋ hormone treatment. The urine samples were collected on the day 7, 21 and 35 respectively after adriamycin injection, and urine protein levels were measured by automatic biochemistry analyzer. The serum protein level of osteoprotegerin （OPG）, nuclear factorKB receptor activating factor ligand （RANKL） were detected by ELISA. Using real-time quantitative PCR and Western blot to measure the expression of OPG and RANKL in tibia tissue. Results compared to the group A, on day7, urinary protein level of group B to E all showed significant increase （P 〈 0. 05） ; compared to group B, day 21 and 35 day, the urinary protein of the C, D and E decreased （P 〈0. 05）. The serum RANKL increased and the OPG decreased in group C compared to B group （P 〈 0. 05 ） ; in both group D and E, the serum OPG increased （P 〈 0. 05 ）, the RANKL level was reduced （P 〈 0. 05） as compared to the C group ; compared to D group, serum OPG in group E reduced, and the RANKL increased （P 〈 0. 05 ）. Compared with the B group, the C group showed a decreased level of protein and mRNA of OPG （ P 〈 0.05, P 〈 0. 01 ） , however, the protein and mRNA of RANKL increased （P 〈0. 01 ） ; compared to the C group, D and E group had an increased levels of both OPG protein and mRNA （P 〈 0. 05 ）, and the RANKL protein and mRNA expression decreased （P 〈 0. 01 ）. Compared with the D group, E group showed decreased level of the OPG protein and mRNA, and increased level of the RANKL protein （P 〈 0. 05, P 〈 0. 01 ）. Conclusions BMSCs may promote osteo

关 键 词：阿霉素肾病 骨质疏松 骨髓间充质干细胞 骨保护蛋白 核因子ΚB受体活化因子配体 

分 类 号：R692[医药卫生—泌尿科学]