自制脂肪乳剂灌胃与普通高脂饲料喂养建立家兔高脂血症动物模型的比较  

Self-made intralipid gastric perfusion versus normal high cholesterol feeding in developing hyperlipidemia rabbit models

作　　者：马建林[1] 马立宁[1] 张家明[2] 张银环[2] 吴明[1] 刘时武[1] 李斌[1] 陈关良[1] 苏哲坦[1] 

机构地区：[1]海南省人民医院心内科,海口570311 [2]华中科技大学同济医学院附属协和医院心内科

出　　处：《中华生物医学工程杂志》2014年第2期125-128,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金（81160036/H0212）;海南省自然科学基金指导性计划项目（806109）;海南省卫生厅科研课题（琼卫2008-37）

摘　　要：目的比较采用自制脂肪乳剂灌胃和普通高脂饲料喂养两种方法建立家兔高脂血症动物模型的效果。方法40只家兔按随机数字表分为自制脂肪乳剂灌胃组（n=20）和高脂饲料喂养组（n=20），分别给予自制脂肪乳剂灌胃连续2周和高脂饲料喂养连续4周。所有动物分别在实验前、后测定血清总胆固醇（TC）、三酰甘油（TG）、载脂蛋白A1（ApoAl）、ApoB，血浆血栓素B2（TXB2）、6-酮-前列腺素F_1α（6-keto-PGF_1α）、内皮素（ET）、红细胞膜总胆醇（M-TC）、红细胞膜流动性（M-Flu）、红细胞丙二醛（E-MDA）、红细胞超氧化物歧化酶（E-SOD）水平，实验结束时取主动脉组织进行病理检查。结果实验后两组家兔TC、TG、ApoB、TXB2、ET、E-MDA水平较实验前升高，ApoAl、6-keto-PGF_1α、M-Flu、E-SOD水平较实验前降低（均P〈0．05）；而M-TC水平差异无统计学意义（均P〉0．05）。实验前、后两组同一指标组间比较，差异均无统计学意义（均P〉0．05）。病理检查显示两组动物主动脉组织均出现动脉粥样硬化早期改变。结论两种方法均可以成功建立高脂血症动物模型，采用自制脂肪乳剂灌胃建立高脂血症动物模型的方法具有可行性。Objective To compare the outcomes of self-made intralipid gastric perfusion with normal high cholesterol feeding in developing hyperlipidemia rabbit models. Methods Forty rabbits were randomly divided into self-made intralipid gastric perfusion group （n=20） and normal high cholesterol feeding group （n=20） based on the random digit table. The rabbits were given self-made intralipid gastric perfusion for 2 weeks and high cholesterol food for 4 weeks respectively. The levels of serum total cholesterol （TC）, triglyceride （TG）, apolipoprotein A1 （ApoA1）, ApoB, plasma thromboxane B2 （TXB2）, 6-keto- prostaglandin F_1α（6- keto- PGF_1α）, endotheline （ET） , erythrocyte membrane total cholesterol （M - TC） , erythrocyte membrane fluidness （M-Flu）, erythrocyte malondialdehyde （E-MDA） and erythrocyte superoxide dismutase （E-SOD） were measured before and after the experiment in all rabbits. The aorta was taken to undergo pathological examination after experiment. Results Compared with baseline, the levels of TC, TG, ApoB, TXB2, ET and E-MDA in two groups of rabbits were increased after the experiment, meanwhile, ApoA1, 6-keto-PGF_1α, M-Flu, E-SOD were decreased （all P〈0.05）, but there were no statistical difference in M-TC level of two groups （both P〉0.05）. There were no statistical difference in any given parameters between two groups before and after the experiment （all P〉O.05） o Pathological examination suggested early atherosclerosis of the aorta in both groups. Conclusions Hyperlipidemia animal models can be successfully established by both ways, suggesting the feasibility to use self-made intralipid gastric perfusion to develop hyperlipidemia animal models.

关 键 词：疾病模型 动物 高脂血症 兔 

分 类 号：R589.2[医药卫生—内分泌]