川芎嗪注射液对自体血回输过程中抗氧化作用  

Antioxidative action of Ligustrazine injection in autologous blood transfusion

作　　者：马武华[1] 蔡诚毅[1] 张子银[1] 王勇[1] 

机构地区：[1]广州中医药大学第一附属医院,510405

出　　处：《中华生物医学工程杂志》2014年第2期154-156,共3页Chinese Journal of Biomedical Engineering

基　　金：广东省科学技术厅社会发展计划项目（20098030801303）;广东省中医药局建设中医药强省科研课题基金（2009397）

摘　　要：目的观探讨川芎嗪在自体血回输对保护细胞免疫功能的抗氧化损伤作用。方法60例择期行脊柱手术患者，ASA分级I～Ⅱ级，术前评估出血量大于400ml，完全随机分两组：试验组和对照组（每组30例）。试验组于收集血液前30min经静脉滴注川芎嗪2mg／kg，在回收血液的肝素盐水和洗涤盐水内加入川芎嗪，终浓度为0．005％；对照组不予静滴川芎嗪，肝素盐水和洗涤盐水内不加川芎嗪。记录术中出血量及回输血量，采集术前（To）及回输血后1h、1d、5d（T1、T2、T3）的静脉血，测定超氧化物歧化酶（SOD）活性和丙二醛（MDA）浓度的变化。结果与To比较，两组患者T1、T2时SOD活性均降低，MDA浓度均升高，T3时试验组SOD比术前高，对照组仍比术前低；试验组MDA基本恢复至术前水平，对照组仍比术前高。组间比较两组SOD、MDA在各时点均差异有统计学意义（P〈0．05）。结论川芎嗪在自体血回输的应用中具有的抗氧化损伤作用。Objective To investigate the action of ligustrazine against oxidative damage for cellular immunoprotection in autologous blood transfusion. Methods Sixty patients scheduled for selective lumbar surgery （ASA I to Ⅱ） , with a pre-surgical estimation of blood loss 〉 400 ml, were recruited. They were randomized into two groups, the study group and the control group （n=30 each）. In the study group, Ligustrazine was given to patients by intravenous dripping at a dose of 2 mg/kg 30 mins before blood collection, and also added to the washing saline and heparinized saline solution （final concentration： 0.005% ） for processing of the collected blood. No Ligustrazine was given to patients or added to the washing saline and heparinized saline solution in the control group. The amount of blood loss and auto-transfusion were measured and recorded. Venous blood samples were collected from all patients before surgery （To） and at 1 hour, 1 and 5 days after （T1, T2 and T3） and measured for the change in SOD activity and MDA level. Results In both groups, the SOD activity was significantly lower and MDA concentration was significantly higher at T1 and T2 as compared to the baseline values at To At T3 SOD activity in the study group was higher but remained a low level in the control group as compared with preoperation, while the MDA in the study group returned to preoperative level but was still higher than baseline in the control group. The SOD activity and MDA level were significantly different between two groups at all time points. （P〈0.05）. Conclusion Ligustrazine has a role against oxidative damage in autotransfusion.

关 键 词：自体血回输 川芎嗪 超氧化物歧化酶 丙二醛 

分 类 号：R285[医药卫生—中药学]