Quantitative genetic analysis of chlorophyll a fluorescence parameters in maize in the field environments  

作　　者：Domagoj ?imi? Hrvoje Lepedu? Vlatka Jurkovi? Jasenka Antunovi? Vera Cesar 

机构地区：[1]Agricultural Institute Osijek [2]Faculty of Humanities and Social Sciences [3]Department of Biology, Josip Juraj Strossmayer University of Osijek

出　　处：《Journal of Integrative Plant Biology》2014年第7期695-708,共14页植物学报（英文版）

基　　金：supported by research grants 073-0731674-1673,073-0731674-0841 and 073‐0730463-0203 from Ministry of Science,Education and Sports,Republic of Croatia

摘　　要：Chlorophyl fluorescence transient from initial to maximum fluorescence （“P”step） throughout two intermedi-ate steps （“J”and“I”） （JIP-test） is considered a reliable early quantitative indicator of stress in plants. The JIP-test is particularly useful for crop plants when applied in variable field environments. The aim of the present study was to conduct a quantitative trait loci （QTL） analysis for nine JIP-test parameters in maize during flowering in four field environ-ments differing in weather conditions. QTL analysis and identification of putative candidate genes might help to explain the genetic relationship between photosynthesis and different field scenarios in maize plants. The JIP-test param-eters were analyzed in the intermated B73 ？ Mo17 （IBM） maize population of 205 recombinant inbred lines. A set of 2,178 molecular markers across the whole maize genome was used for QTL analysis revealing 10 significant QTLs for seven JIP-test parameters, of which five were co-localized when combined＆amp;nbsp;over the four environments indicating polygenic inheritance and pleiotropy. Our results demonstrate that QTL analysis of chlorophyl fluorescence parameters was capable of detecting one pleiotropic locus on chromosome 7, coinciding with the gene gst23 that may be associated with efficient photosynthe-sis under different field scenarios.Chlorophyl fluorescence transient from initial to maximum fluorescence （“P”step） throughout two intermedi-ate steps （“J”and“I”） （JIP-test） is considered a reliable early quantitative indicator of stress in plants. The JIP-test is particularly useful for crop plants when applied in variable field environments. The aim of the present study was to conduct a quantitative trait loci （QTL） analysis for nine JIP-test parameters in maize during flowering in four field environ-ments differing in weather conditions. QTL analysis and identification of putative candidate genes might help to explain the genetic relationship between photosynthesis and different field scenarios in maize plants. The JIP-test param-eters were analyzed in the intermated B73 ？ Mo17 （IBM） maize population of 205 recombinant inbred lines. A set of 2,178 molecular markers across the whole maize genome was used for QTL analysis revealing 10 significant QTLs for seven JIP-test parameters, of which five were co-localized when combined＆amp;nbsp;over the four environments indicating polygenic inheritance and pleiotropy. Our results demonstrate that QTL analysis of chlorophyl fluorescence parameters was capable of detecting one pleiotropic locus on chromosome 7, coinciding with the gene gst23 that may be associated with efficient photosynthe-sis under different field scenarios.

关 键 词：Chlorophyll a fluorescence parameters field conditions intermated B73× Mo17 recombinant inbred lines JIP-TEST maize （Zeamays L.） quantitative trait loci 

分 类 号：S513[农业科学—作物学]