金雀异黄酮促进体外培养的大鼠股骨组织代谢和抑制骨吸收  

Genistein promoted metabolism and inhibited resorption of rats' femur tissue invitro

作　　者：周建[1] 陈克明[1] 葛宝丰[1] 马小妮[1] 成魁[1] 高玉海[1] 闫丽娟[1] 石文贵[1] 

机构地区：[1]中国人民解放军区兰州军区兰州总医院骨科研究所,甘肃兰州730030

出　　处：《中国医院药学杂志》2014年第17期1460-1464,共5页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究金雀异黄酮对体外培养股骨组织(骨干和骨骺端)吸收活性和骨代谢的影响。方法:体外分离培养大鼠股骨组织分别培养骨干和骨骺端48 h后采用终浓度为1×10-5 mol·L-1的金雀异黄酮对体外培养骨干和骨骺端进行处理;测定抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP)、培养基中葡萄糖(glucose,Glu)、乳酸(lactic acid,La)、Real-Time RT-PCR抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,StrACP),集落刺激因子(macrophage colony stimulating factor,MCSF),组织激酶K(cathepsin K,CTSK)。结果:1×10-5 mol·L-1金雀异黄酮可抑制StrACP活性,增加培养基中La含量和减少培养基中葡萄糖含量,抑制StrACP,MCSF,CTSK mRNA的表达。结论:金雀异黄酮抑制体外培养大鼠股骨组织的骨骺端和骨干吸收活性和促进其代谢活性。OBJECTIVE To investigate the effects of genistein on metabolism of femoral bone tissue and resorption activity of rats in vitro. METHODS Femoral tissues were isolated and cultured in vitro from rats. The femoral tissue was treated with final concentration of 1 x 10-5 mol·L-1 genistein on culture in vitro. Tartrate-resistant acid phosphatase（StrACP）, glucose content and lactic acid were determined, StrACP, MCSF and CTSK mRNA were detected by Real-Time RT-PCR. RESULTS 1 x 10-5 mol·L-1 genistein could inhibit the activity of StrACP and decreased the content of glucose and increased lactic acid of medium, inhibited StrACP, MCSF and CTSK mRNA expression. CONCLUSION Genistein inhibited resorption of bone and promoted femoral tissue metabolic activity.

关 键 词：金雀异黄酮 股骨组织 抗酒石酸酸性磷酸酶 

分 类 号：R932[医药卫生—生药学]