多巴胺与鲱鱼精DNA相互作用的电化学研究  

Electrochemical study of the interaction between dopamine and herring sperm DNA

作　　者：王娟[1] 

机构地区：[1]菏泽学院食品分析与检测重点实验室,山东菏泽274015

出　　处：《云南大学学报（自然科学版）》2014年第5期750-754,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(21105023)

摘　　要：通过循环伏安法制备了聚L-半胱氨酸修饰电极,利用该修饰电极研究了Cu2+存在下多巴胺(DA)与鲱鱼精DNA在Tris-马来酸缓冲溶液中的相互作用.结果表明:在pH7.0的Tris-马来酸缓冲溶液中,DA在聚L-半胱氨酸复合膜修饰电极上有一对明显的氧化还原峰,加DNA后氧化还原峰电流明显下降,说明DNA和DA能发生相互作用.再加入Cu2+后氧化还原峰电流减小更大,说明Cu2+对DA和DNA的相互作用有较大影响.Poly L- cysteine modified electrode was prepared by cyclic vohammetry method, with which the interaction between dopamine （DA） and herring sperm DNA with the presence of copper ion in the Tris - maleic acid buffer solution was studied. Result showed that there was an obvious redox peak of DA on the poly L - cyste- ine composite film modified electrode in the Tris - maleic acid buffer solution of pH 7.0. After adding the DNA, the redox peak decreased obviously. This of DA decreased further as adding coppe DA and DNA.

关 键 词：多巴胺 鲱鱼精DNA CU2+ 循环伏安法 

分 类 号：O614.41[理学—无机化学]