新生儿缺氧缺血性脑病患儿血液中血管内皮生长因子和miR-125b表达水平及相关性研究  

Relationship between vascular endothelial growth factor and miR-125b in neonates with hypoxic-ischemic encephalopathy

作　　者：黄群[1] 谭丹凤[1] 马敏[1] 黄广文[1] 龙小兰[1] 吴虹[1] 

机构地区：[1]湖南省妇幼保健院儿童保健科,长沙410008

出　　处：《临床与病理杂志》2014年第4期353-357,共5页Journal of Clinical and Pathological Research

摘　　要：目的:探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)和miR-125b在新生儿缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)患儿和正常新生儿血液中表达水平及相关性。方法:收集20例正常新生儿和20例HIE患儿血液标本,分离血浆和外周血单个核细胞(peripheral blood mononuclear cell,PBMC),利用real-time PCR检测PBMC中miR-125b表达,ELISA方法检测血浆中VEGF含量。结果:与正常对照组相比,HIE患儿PBMC中miR-125b表达显著降低(P=0.00002),而血浆中VEGF水平显著升高(P=6.26×10–8);同时在正常组中,miR-125b表达与VEGF水平无相关性(r=0.016,P=0.946,n=20),但在HIE组(r=–0.505,P=0.023,n=20)二者却呈显著负相关。结论:新生儿脑缺血缺氧时可能通过降低miR-125b表达从而升高VEGF表达。Objective： To explore the expression attd correlation between vascular endothelial growth factor （VEGF） and miR-125b in healthy and hypoxic-ischemic encephalopathy （HIE） neonatus. Methods： A total of 20 controls and 20 HIE samples were recruited in this study, plasma and peripheral blood mononuclear cell （PBMC） were prepared for detecting the miR-125b expression and VEGF concentration using real-time PCR and ELISA kit, respectively. Result： Compared with controls, miR-12Sb expression in PBMC was significantly decreased （P=0.00002） but VEGF concentration in plasma was increased in HIE group （P=6.26×10-8）. Meanwhile, there was no significant correlation between miR-125b expression and VEGF level in controls （r=0.016, P=0.946,n=20）. However, significant correlation was observed in HIE group （r=-0.505, P=0.023, n=20）. Conclusion： The VEGF level was increased possibly due to the down-regulation ofmiR-125b in HIE neonatus.

关 键 词：新生儿缺氧缺血脑病 微小RNA 血管内皮生长因子 

分 类 号：R473.72[医药卫生—护理学]