孕期注射Poly（I∶C）对大鼠子代认知行为及脑髓鞘碱性蛋白的影响  

Studies on the cognitive behavior and the myelin basic protein of the offspring in adult by infecting polyriboinosinic-polyribocytidilic acid in pregnacy

作　　者：孟凡翠[1] 李文强[1] 李雪[2] 卢艳梨 丁爽[1] 张红星[1] 吕路线[1] 

机构地区：[1]新乡医学院第二附属医院,河南省生物精神病学重点实验室,新乡453002 [2]郑州大学第一附属医院精神医学科

出　　处：《中华行为医学与脑科学杂志》2014年第8期728-731,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81371472）,河南省基础与前沿研究项目（112300413226）,河南省科技杰出创新人才项目（124200510019）,新乡医学院重点学科开放课题（ZD200991）

摘　　要：目的 通过分析孕鼠感染多聚肌苷酸：多聚胞苷酸（Polyriboinosinic-polyribocytidilic acid,Poly （I∶C）后子代行为学和脑白质髓鞘碱性蛋白（myelin basic protein,MBP）的变化,了解脑白质在孕期感染所致子代行为异常中的作用.方法两组模型组孕鼠分别给予5 mg/kg Poly（I∶C）和10 mg/kgPoly（I∶C）处理,对照组给予5 mg/kg生理盐水处理.待子代生长至8周时用前脉冲抑制实验、被动规避实验、主动规避实验检测子代精神分裂症样行为,用免疫组化方法观察脑白质MBP的变化.结果 前脉冲抑制结果显示PP2、PP4、PP8差异有显著性（F=10.381,P=0.001,F=10.313,P=0.001,F=15.233,P=0.000）,两个模型组均小于对照组,双倍剂量组均小于单倍剂量组（P＜0.05）;被动规避结果显示T1、T2差异有显著性（F=17.524,P=0.000,F=23.555,P=0.000）,两个模型组的T1时间均大于对照组,双倍剂量组大于单倍剂量组（P＜0.05）;两模型组T2时间均小于对照组,双倍剂量组小于单倍剂量组（P＜0.05）;主动规避结果显示总条件反射次数和条件反射率差异有显著性（F=8.631,P=0.000,F=6.986,P=0.001）,两个模型组均小于对照组,双倍剂量组小于单倍剂量组（P＜0.05）;两模型组大鼠脑白质MBP免疫组化结果与对照组相比有统计学意义（P＜0.05）,两模型组比较差异无统计学意义（P＞0.05）.结论 Poly（I∶C）处理后子鼠存在行为学和MBP的改变,且行为学改变的程度与药物剂量有相关性.Objective By analyzing the changes in behavior and the myelin basic protein （MBP） of the offspring in adult that treated with Poly（I∶C） during pregnancy,and to understand the role of white matter abnormalities in the abnormal behavior of the offspring induced by infection in maternal hosts.Methods Two models maternal female rats were given Poly（I∶ C） with 5 mg/kg and 10mg/kg respectively during the early pregnancy,and control maternal female rats was administered 5 mg/kg saline.The prepulse inhibition test,passive avoidance test and active avoidance test were used to evaluate schizophrenia like behaviors for each groups offspring in 8 weeks,and the expression of MBP was detected by immunohistochemical staining methods.Results The results of prepulse inhibition test showed that significant differences of PP2,PP4 and PP8 results existed among control group,single-dose model group and double-dose model group （F=10.381,P=0.001,F=10.313,P=0.001,F=15.233,P=0.000）.Compared with the control group,the two model groups showed significantly lower,the double-dose model group was lower than single-dose model group （P＜0.05）.In passive avoidance test,there were significant differences of T1 and T2 results existed among control group,single-dose model group and double-dose model group （F=23.555,P=0.000,F=17.524,P=0.000）.The T1 results of two model groups were significantly higher than control group,the double-dose model group was significantly higher than single-dose model group （P＜0.05） ; the T2 results of two model groups were lower than control group,the double-dose model group was lower than single-dose model group（P＜0.05）.The results of passive avoidance test indicated that significant differences existed among control group,single-dose model group and double-dose model group in whole period of testing and total conditioned response rate（F=8.631,P=0.000,F=6.986,P=0.001）,the two model groups were significantly lower than control group,double-dose model group was significantly low

关 键 词：精神分裂症 大鼠 多聚肌苷酸 多聚胞苷酸 脑白质 髓鞘碱性蛋白 

分 类 号：R715.3[医药卫生—妇产科学]