海洋放线菌 Y18的分子鉴定、生物活性及其抗菌物质发酵条件优化  

MOLECULAR IDENTIFICATION,ANTIMICROBIAL ACTIVITY AND FERMENTATION CONDITIONS OF ANTIBIOTIC SUBSTANCES OF MARINE ACTINOBACTERIA NOCARDIOPSIS SP. Y18

作　　者：苑孟 俞勇[2] 李会荣[2] 董宁[1,2] 张晓华[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]国家海洋局极地科学重点实验室中国极地研究中心,上海200136

出　　处：《极地研究》2014年第3期292-299,共8页Chinese Journal of Polar Research

基　　金：国家自然科学基金项目(41276175);上海市自然科学基金项目(11ZR1441000)资助

摘　　要：从北冰洋楚科奇海海洋沉积物中分离到一株放线菌Y18,其良好生长需要海水。经16S rRNA基因序列分析确定该菌株属于拟诺卡氏菌属(Nocardiopsis),次级代谢产物合成酶基因及抗菌活性筛选发现,它含有聚酮类Ⅱ型(PKSⅡ)和非核糖体含硫多肽类(non ribosome-bound sulfur-containing peptides,NRPS)化合物合成酶基因,并且对枯草芽孢杆菌和白色念珠菌有抑制作用。以枯草芽孢杆菌为指示菌对Y18的发酵条件进行了初步优化,适合的发酵条件为:发酵培养基(蛋白胨2 g,酪素水解物2 g,酵母膏2 g,葡萄糖1 g,海水1 L),种子液接种量4%,发酵温度28℃,发酵培养基初始pH值8.0,培养时间6天,1 000 mL三角瓶装液量20%。并对Y18发酵液稳定性进行了测定,结果显示:发酵液对酸碱、热的稳定性都较强,4℃下贮藏发酵液的抗菌活性保留时间较长。The actinomycete strain Y18 was isolated from ocean sediments collected from the Chukchi Sea , Arctic Ocean. Its good growth needs sea water .It belongs to genus Nocardiopsis according to 16S rRNA sequence analysis .The results of secondary-metabolite biosynthesis genes screening show it contains Polyethylene ketone type Ⅱ（ PKSⅡ） and non ribosome-bound sulfur-containing peptides （ NRPS） genes.It has activities against both Bacillus subtilis and Candida albicans.The optimal fermentation conditions of Y 18 against Bacillus subtilis are as follows： the fer-mentation medium containing peptone 2 g, casein hydrolysate 2 g, yeast extract 2 g, glucose 1 g, sea water 1 L, the seed liquid inoculum for 4%, medium initial pH8.0, the culture temperature of 28℃, liquid volume 20%in 1 000 ml flask, and fermentation time 6 days.Through the research of stability of heat , acid, alkali and storage we found that it had a stable activity to heat , acid, alkali and storage at 4℃.

关 键 词：海洋放线菌 分子鉴定 抗菌活性 发酵条件 北冰洋 

分 类 号：S917.1[农业科学—水产科学]