系统性红斑狼疮患者自身抗体联合检测的临床意义  

Clinical significance of combined detection of autoantibodies in patients with systemic lupus erythematosus

作　　者：杨媛慧[1] 周薇[1] 陆红[1] 谢闻悦[1] 

机构地区：[1]北京大学深圳医院检验科,广东深圳518036

出　　处：《检验医学与临床》2014年第17期2380-2381,共2页Laboratory Medicine and Clinic

基　　金：广东省深圳市科委科技计划项目(201203031)

摘　　要：目的探讨3种抗体联合检测诊断系统性红斑狼疮(SLE)的临床意义。方法选择2012年1月至2014年1月本院收治的SLE患者60例(SLE组),以同期于本院体检健康者60例作为对照组。采用酶联免疫吸附法检测抗双链DNA(dsDNA)抗体,采用免疫印迹法检测抗核小体抗体(AnuA)、抗Sm抗体,比较3种抗体单独检测及联合检测的诊断效能。结果 SLE组AnuA、抗Sm抗体、抗dsDNA抗体及3种抗体联合检测阳性率分别为93.3%、33.3%、46.7%、96.7%,均高于对照组检测结果(P<0.05)。3种抗体联合检测具有更高的SLE诊断效能。结论 AnuA、抗ds-DNA抗体、抗Sm抗体联合检测有助于避免SLE的漏诊,从而达到早期诊断、治疗的目的。Objective To explore the clinical significance of combined detection of autoantibodies in patients with systemic lupus erythematosus （SLE） .Methods A total of 60 SLE patients ,treated in this hospital from Jan . 2012 to Jan .2014 ,were enrolled as SLE group ,and 60 healthy subjects were enrolled as control group .Enzyme-linked immunosorbent assay was performed to detect anti-double stranded DNA （dsDNA ） antibody ,and Western blot was used to detected ant-nucleosome antibody （AnuA） and anti-Sm antibody .Diagnostic performance of single and com-bined detection of antibodies was compared .Results Positive rates of anti-dsDNA antibody ,AnuA ,anti-Sm antibody and combined detection of the three antibodies were 93 .3% ,33 .3% ,46 .7% and 96 .7% in SLE group ,which were significantly higher than control group（P〈0 .05） .Combined detection of the three antibodies were with higher diag-nostic performance .Conclusion Combined detection of AnuA ,anti-Sm antibody and anti-dsDNA antibody might be helpful to avoid missed diagnosis of SLE .

关 键 词：系统性红斑狼疮 抗核小体抗体 抗SM抗体 抗双链DNA抗体 联合检测 

分 类 号：R758.62[医药卫生—皮肤病学与性病学]