淫羊藿配合髓芯减压术治疗兔激素性股骨头坏死的实验研究  

The experimental study of therapeutic effect of epimedium combined with core decompression on hormonal femoral head necrosis in rabbits

作　　者：刘志国[1] 马文海[1] 李杰[1] 

机构地区：[1]河北省保定市第一中心医院,071000

出　　处：《河北医药》2014年第19期2894-2897,共4页Hebei Medical Journal

摘　　要：目的观察淫羊藿配合随芯减压术对兔激素性股骨头坏死的治疗效果并探讨其作用机制。方法 60只新西兰大白兔随机分为对照组(n=12)和实验组(n=48)。用改进的马血清加甲基强的松龙的方法诱导制备兔激素性股骨头坏死动物模型。根据治疗方法不同,将模型动物随机分为模型组、手术组(髓芯减压术)、中药组(淫羊藿)、综合组(随芯减压术+淫羊藿)。治疗后对5组股骨头的骨密度、生物力学、IGF-1、BMP-2 mRNA和蛋白的表达进行检测。结果与对照组比较,模型组骨密度及抗压力明显下降(P<0.05),中药组、手术组、综合组均有所改善(P<0.05),综合组改善效果最好(P<0.05)。IGF-1mRNA和蛋白水平,模型组高于对照组(P<0.05)。BMP-2 mRNA和蛋白水平,模型组较对照组明显下降(P<0.05)。IGF-1、BMP-2 mRNA和蛋白的表达,中药组与综合组,手术组与模型组比较,差异无统计学意义(P>0.05);而在中药组和综合组的表达显著高于模型组和手术组(P<0.05)。IGF1与BMP-2 mRNA及蛋白在股骨头的表达呈明显正相关(P<0.05)。结论淫羊藿通过上调IGF1和BMP-2的表达而促进激素性股骨头坏死的损伤修复,其与随芯减压术相结合可有效增加股骨头骨密度,提高骨强度,为临床上治疗激素性股骨头坏死提供了一种较为有效的方法。Obj ectiev To investigate the therapeutic effect of epimedium combined with core decompression on hormonal femoral head necrosis in rabbits ,and to explore its action mechanism .Methods Sixty New Zealand white rabbits were randomly divided into control group （ n =12 ） and experimental group （ n =48 ） .The animal models with hormonal femoral head necrosis were established by the modified method of horse serum plus methylprednisolone , then according to different therapeutic methods the model animals were divided into model group , operation group （ core decompression ） , traditional Chinese medicine （ TCM ） group （ epimedium ） , integration group （ core decompression ＋epimedium ） .After 10-week treatment,the bone mineral density,biomechanics of femoral head were examined ,and the expression levels of IGF1, BMP2 mRNA and protein were detected by RT-PCR and Western Blot .Results As compared with those in control group ,the bone mineral density and biomechanical properties of femoral head were significantly decreased in model group , which were obviously improved in TCM group,operation group and integration group （ P 〈0.05）,especially in integration group （ P 〈0.01）.The expression levels of IGF1 mRNA and protein in model group were significantly higher than those in control group （P 〈0.05）.The expression levels of BMP-2 mRNA and protein in model group were obviously decreased ,as compared with those in control group （ P 〈0.05）.However there were no significant differences in the expression levels of IGF 1,BMP-2 mRNA and protein between TCM group and integration group and between operation group and model group （ P 〉0.05）.The expression levels of IGF1,BMP-2 mRNA and protein in TCM group and integration group were significantly higher than those in model group and operation group （ P 〈0.05）.The expression of IGF1 in femoral head was positively correlated to that of BMP-2 mRNA and protein （ P 〈0.01）.Conclusion Epimedium plays an important role in repara

关 键 词：激素性股骨头坏死 淫羊藿 随芯减压术 IGF-1 BMP-2 

分 类 号：R285.5[医药卫生—中药学]