一株异养硝化细菌的分离鉴定及最佳固定化条件的选择  

Isolation and Identification of a Heterotrophic Nitrifier and Its Optimal Conditions for Immobilization

作　　者：范宝芸[1] 杨杰勇[2] 吕永康[2] 刘玉香[3] 

机构地区：[1]太原理工大学体育学院,太原030024 [2]太原理工大学煤科学与技术教育部和山西省重点实验室,太原030024 [3]太原理工大学环境科学与工程学院,太原030024

出　　处：《太原理工大学学报》2014年第4期468-472,505,共6页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金资助项目(51078252);山西省国际合作计划项目(2010081018);山西省自然科学基金资助项目(2010011016-1)

摘　　要：采用传统的微生物分离纯化方法,从焦化废水活性污泥中分离出一株异养硝化细菌Y1,菌落为白色、半透明。经形态、生理生化特性以及16SrRNA基因序列分析,初步鉴定该菌属于不动杆菌属(命名为Acinetobacter sp.Y1)。研究了不同包埋材料的包埋法固定化异养硝化细菌的脱氮能力。通过实验结果分析,得出当以PVA(聚乙烯醇)+CA(海藻酸钠)为包埋载体制作固定化小球时降解效果最佳,实验培养5d后,氨氮剩余量为17.68mg/L,达到国家二级排放标准,总氮去除量也达到64.17%.Strain Y1with high biodegradation activity of ammonium was isolated from the activated sludge and ammonium-contaminated soils in Taiyuan of Shanxi.The colonies were translucent white.DNA sequencing and homologous analysis of 16SrRNA gene identified that Y1belonged to an Acinetobacter sp.Strain with high biodegradation activity of ammonium using embedding methods was experimentally investigated.The results indicate that immobilized ball have best degradation when using PVA（polyvinyl alcohol）＋CA（sodium alginate）as embedding carrier,culturing the strain 5d,surplus capacity of ammonia nitrogen is 17.68mg/ml,reaching the national emission standards for grade two,the removal rate of total nitrogen can reach 64.17%.

关 键 词：异养硝化细菌 分离 固定化 载体 

分 类 号：TQ701[化学工程]