哈茨木霉LTR-2对椒样薄荷耐盐生理特性的影响  

Impacts of Trichoderma harzianum LTR-2 on salt-tolerant physiological characteristics of peppermint (Mentha piperita L.)

作　　者：李哲[1] 郭凯[1] 吴晓青[1] 陈泉[1] 李纪顺[1] 杨合同[1,2] 

机构地区：[1]山东省科学院生物技术研究中心,山东省应用微生物重点实验室,山东济南250014 [2]山东理工大学生命科学学院,山东淄博250049

出　　处：《山东科学》2014年第4期17-24,41,共9页Shandong Science

基　　金：山东省科学院青年科学基金(2013QN012);国际科技合作项目(2011DFA30990);国际科技合作项目(2007DFA30630)

摘　　要：木霉菌(Trichoderma spp.)是一类能够刺激作物生长并增强其抵抗生物和非生物胁迫能力的多功能益生真菌,目前国内外对于生防木霉菌在增强植物耐盐性及其作用机理方面尚缺乏深入、系统的研究。本文研究了生防木霉菌LTR-2对椒样薄荷耐盐生理特性的影响,结果显示,在200 mmol/L NaCl胁迫下,LTR-2有效缓解了盐胁迫对椒样薄荷的生长和光合的抑制。探讨了LTR-2接种的椒样薄荷耐受NaCl胁迫的生理生化基础,结果表明,LTR-2提高了椒样薄荷的抗氧化活力,减少了活性氧积累;增强了细胞膜和液泡膜H+-ATPase以及Na+/H+逆向转运蛋白等离子转运通道的活性,从而调节盐分的吸收和分布。这些结果为椒样薄荷在盐碱地区的引种及木霉菌剂的开发应用提供了实验依据。Trichoderma spp.is a kind of multi-functional probiotics fungus that can stimulate crop growth and increase its capability against abiotic and biotic stresses.However,advanced and systematic global research is very limited in the areas of its plant salt tolerance increase and function mechanism.This paper addresses the impacts of Trichoderma harzianum LTR-2 on NaCl tolerant physiological characteristics of peppermint （Mentha piperita L.）.Results show that LTR-2 can effectively alleviate growth inhibition and photosynthetic damage of peppermint under 200 mmol/L NaCl stress.The paper also investigates the physiological and biochemical basis of NaCl stress tolerance of LTR-2 inoculated peppermint.Results indicate that LTR-2 can increase antioxidant activity of peppermint and reduce ROS accumulation.It can also increase the activities of plasma membrane （PM） and tonoplast H ＋-ATPase and Na ＋/H ＋ antiporter and then regulate salt absorption and distribution.These results provide experimental basis for the development and application of peppermint and LTR-2 in saline soil.

关 键 词：NACL胁迫 椒样薄荷 哈茨木霉LTR-2 耐盐性 

分 类 号：Q945.78[生物学—植物学]