甘草酸标准样品的研制  

Preparation of certified reference materials of glycyrrhizic acid

作　　者：王岱杰[1] 周晓晶 王尉 于金倩[1] 王晓[1] 李强[1] 耿岩玲[1] Dan Staerk 

机构地区：[1]山东省分析测试中心,山东济南250014 [2]北京理化分析测试中心,北京100089

出　　处：《山东科学》2014年第4期25-32,共8页Shandong Science

基　　金：国家质检公益性行业科研专项(201310243);山东省海外高层次人才资助专项资金;北京市市级财政专项(PXM2014_178305_000007_00136778)

摘　　要：建立了甘草酸标准样品的研制方法。采用硅胶柱色谱结合重结晶方法对甘草酸进行分离制备,UV、IR、MS和NMR等方法对甘草酸进行结构表征,并进行了均匀性、稳定性检验,经国内8个具有分析资质的实验室进行协同定值,甘草酸标准样品的标准值为99.29%,置信度95%的不确定度值为0.12%。该标准样品达到国家标准样品的技术要求,可用于有关甘草酸检测方法的校正和相关产品的质量控制。We established a preparation approach of certified reference material of glycyrrhizic acid.Glycyrrhizic acid was separated by silica gel column chromatography and recrystallization,whose chemical structure was denoted by UV,IR,MS and NMR.Its homogeneity and stability test were also systematically checked.Eight qualified domestic laboratories cooperatively certified its value.Results show that its certified value is 99.29％,and the expanded uncertainty with 95％ confidence coefficient is 0.12％.The reference material satisfied the technical requirement of domestic certified reference material,so it could be used for the validation of glycyrrhizic acid detection method and quality control over relative products.

关 键 词：甘草酸 标准样品 均匀性 稳定性 定值 不确定度 

分 类 号：R284.1[医药卫生—中药学]