An Evaluation of Genotoxicity and Cytotoxicity of Melamine in Combination with Cyanuric Acid at Three Mass Ratios  

作　　者：LIU Xin HUANG Da Wei WU Ke Jia WU Yong Ning JIA Xi Wu GONG Zhi Yong 

机构地区：[1]Institute of Food Science and Engineering, Wuhan Polytechnic University [2]Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan Polytechnic University [3]China National Center for Food Safety Risk Assessment, Chaoyang District

出　　处：《Biomedical and Environmental Sciences》2014年第8期641-645,共5页生物医学与环境科学（英文版）

基　　金：supported by a grant from the National Basic Research Program of China(2012CB720804);the Research Special Fund for Public Welfare Industry of Health(NO.200902009);the Wuhan Science and Technology Bureau under Scientific and technological project(NO.201070934341)

摘　　要：Melamine in combination with cyanuric acid has been considered to be more toxic than either melamine or cyanuric acid alone. The objective of this study was designed to evaluate the combined genotoxicity and cytotoxicity of melamine （M） and cyanuric acid （C） at three mass ratios （1：1, 1：2, 2：1）. MC （1：1）, MC （1：2）, and MC （2：1） were evaluated for their potential genotoxic risk, at gene level by Ames test, and at chromosomal level by micronucleus test. In order to evaluate cytotoxicity in HEK-293 cells, the MTT assay was included. Western blot was also employed to investigate the renal injury molecule-1 （Kim-1） expression in HEK-293 cells exposed to MC. Neither genotoxicity at gene level nor at chromosomal level was observed for MC （1：1）, MC （1：2）, and MC （2：1）. Based on MTT assay, three ratios of MC at 82.5 and 165 μg/mL slightly inhibited viability of HEK-293 cells （P〈0.05）. MC （1：1） at 41.25 and 82.50 IJg/mL could elevate the Kim-1 expression in HEK-293 cells.Melamine in combination with cyanuric acid has been considered to be more toxic than either melamine or cyanuric acid alone. The objective of this study was designed to evaluate the combined genotoxicity and cytotoxicity of melamine （M） and cyanuric acid （C） at three mass ratios （1：1, 1：2, 2：1）. MC （1：1）, MC （1：2）, and MC （2：1） were evaluated for their potential genotoxic risk, at gene level by Ames test, and at chromosomal level by micronucleus test. In order to evaluate cytotoxicity in HEK-293 cells, the MTT assay was included. Western blot was also employed to investigate the renal injury molecule-1 （Kim-1） expression in HEK-293 cells exposed to MC. Neither genotoxicity at gene level nor at chromosomal level was observed for MC （1：1）, MC （1：2）, and MC （2：1）. Based on MTT assay, three ratios of MC at 82.5 and 165 μg/mL slightly inhibited viability of HEK-293 cells （P〈0.05）. MC （1：1） at 41.25 and 82.50 IJg/mL could elevate the Kim-1 expression in HEK-293 cells.

关 键 词：An Evaluation of Genotoxicity and Cytotoxicity of Melamine in Combination with Cyanuric Acid at Three Mass Ratios TA 

分 类 号：R114[医药卫生—卫生毒理学]