“神舟八号”飞船实时水稻细胞培育及表达谱芯片分析  

Growth of Rice Cells in Shenzhou 8 under Microgravity and Transcriptome Analysis

作　　者：金晶[1] 陈海莹[1] 蔡伟明[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《载人航天》2014年第5期480-485,490,共7页Manned Spaceflight

基　　金：科技部973项目(2011CB710902)

摘　　要：成功建立了在空间微重力条件下水稻细胞的培养体系,并在"神舟八号"飞船空间飞行时的微重力条件下对培养过程中的细胞进行了实时固定,保存了水稻细胞中反映微重力条件下基因表达状态的RNA样品。返地后,用RNA质量检测和表达谱芯片方法对回收样品进行了分析。提取的水稻细胞RNA质量检测合格,表明本次空间的实验和固定方法可行和可靠。进一步分析水稻表达谱变化情况显示,微重力条件下水稻细胞的表达谱相比地面重力条件和微重力条件下人工1 g条件下的对照有显著差异。Culture system of rice cells under spatial microgravity conditions was successfully estab-lished in this study .Moreover , a real-time fixation of cultured cells was undertaken to get the RNA samples which reflected the gene expression pattern of rice cells under microgravity conditions .After Shenzhou 8 spaceship returned to earth , collected samples were analyzed by RNA quality-test and micro-array analysis .The results of RNA quality-test indicated that the experimental and fixation methods in space were feasible and reliable .Furthermore, analysis of gene expression changes showed that the pattern of gene expression in rice cells under microgravity was significantly different as compared with ground gravity conditions and artificial 1 g conditions .

关 键 词：神舟八号飞船 水稻 细胞 基因 基因芯片 单项匹配实验 整合匹配实验 力学实验 

分 类 号：V524.1[航空宇航科学与技术—人机与环境工程] Q693[生物学—生物物理学]