112例妊娠早期自然流产患者绒毛细胞培养及核型分析  

Villus cell culture and its karyotype analysis for 112 women who had spontaneous abortion in early pregnancy

作　　者：李远眺[1] 邹燕[1] 李勇[1] 

机构地区：[1]广西医科大学第四附属医院检验科遗传室,广西柳州545005

出　　处：《国际检验医学杂志》2014年第17期2319-2320,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨绒毛细胞染色体异常与妊娠早期自然流产间的临床关系。方法对112例妊娠早期自然流产患者绒毛细胞进行短期培养、染色体制备及核型分析。结果成功检测绒毛标本105例,占93.75%(105/112)。其中,检出正常核型50例,占47.62%(50/105);异常核型55例,占52.38%(55/105)。异常核型中以数目异常为主,其中三体型最为常见(33例),三倍体6例,四倍体及复合三体各1例,嵌合体2例,性染色单体1例,结构异常6例,另有染色体正常变异5例;正常核型中男性20例,女性30例。结论胚胎染色体异常是导致妊娠早期自然流产的重要因素之一,临床上开展流产绒毛染色体检查有利于判断本次流产原因,合理指导下次妊娠。Objective To investigate the clinical relationship between villus cells chromosome abnormality and spontaneous a-bortion in early pregnancy.Methods Villus cells from 112 women who just had spontaneous abortion in early pregnancy were cul-tured and prepared for karyotype analysis.Results 105 specimens were anaIyzed successfully which accounted for 93.75%（105/112）.Among them 50 specimens were normal karyotype which accounted for 47.62%（50/105）,55 cases were abnormal karyotype accounted for 52.38%（55/105）.Abnormal number was the major type of abnormal karyotype.Among them there were 33 cases of trisomy which were most common,6 cases of triploid,1 case of tetraploid and 1 case of compound,2 cases of chimera,1 case of sex-ual chromatids and 6 cases of structural abnormalities.In the specimens with normal karyotype,20 cases were from male,30 cases were from female.Conclusion Embryo chromosome abnormality is one of the factors causing early pregnancy spontaneous abor-tion.Conduct villus chromosome examination in clinical treatment is useful to determine the reasons of abortion and to guide the next pregnancy.

关 键 词：绒毛细胞 核型分析 自然流产 

分 类 号：R714.21[医药卫生—妇产科学]