高效功能菌株SRBWN3的分离筛选及脱硫特性研究  

The Isolation of a Highly Efficient Function Strain SRBWN3 and a Study of Its Desulphurisation Characteristics

作　　者：肖利萍[1] 汪兵兵[1] 魏芳[1] 裴格[1] 任军俊[1] 

机构地区：[1]辽宁工程技术大学建筑工程学院

出　　处：《上海环境科学》2014年第4期143-147,共5页Shanghai Environmental Sciences

基　　金：国家自然科学基金项目,编号:51174267;辽宁省“百千万人才工程”项目,编号:2009921106;辽宁省高校科研立项计划项目,编号:2008300

摘　　要：自污水处理厂的回流污泥和垃圾处理厂的垃圾渗滤液中分离纯化出8株硫酸盐还原菌，从这8株菌中筛选出还原SO4^2-能力最强的高效功能菌株SRBWN3o经鉴定，该菌株形态呈杆状或弧状，革兰氏染色阴性、芽孢染色阴性，单极生鞭毛，初步认定为脱硫弧菌属。并进行了单因素实验，进一步探讨了SRBWN3脱硫特性及最适宜条件。结果表明，该菌株脱硫的最佳pH值为7、最佳C：S比为1．5，最佳Fe2｜浓度为150mg／L；在优化条件下，脱硫率最高，反应7d时SO4^2-去除率达到84．3％。因此，从回流污泥中分离．筛选得到的SRBWN3。是高效脱硫功能菌株，其反应条件易于控制，可为实际应用提供参考。Of eight sulphate-reducing bacteria （SRB） strains isolated and purified from the sludge of a sewage treatment plant and the garbage leachate of a waste treatment plant, a highly effective SRBWN3 strain of reducing sulphates was screened out. The SRBWN3 strain was rod- or vibrioid-shaped, Gram-negative, non-spore-forming, dyed by monopolar flagella, and was preliminarily identified to be desulfovibrio. A series of single-factor tests were carried out to further find out the desulphurisation characteristics of SRBwN3 and its most appropriate conditions. Results indicated that the preferable pH value was 7, ratio of C to S was 1.5, and Fe2＋ content was 150 mg/L. Under such optimal conditions, the uppermost desulphurisation could be achieved to be 84.3% removal of sulphates after reacting for seven days. It concluded that the SRBWN3 was a highly effective strain isolated and screened from the sewage sludge and could be easily controlled as well as put to practical use.

关 键 词：硫酸盐还原菌(SRB) 分离纯化 筛选 高效功能菌株 脱硫弧菌 脱硫特性 

分 类 号：X791.03[环境科学与工程—环境工程]