组织工程重建修复犬膀胱后对上尿路的影响  

Effects of tissue engineering bladder reconstruction in canines on upper urinary tract

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作  者:郭宇[1] 陈伟[1] 贾维胜[1] 马超[1] 侯祥麟[2] 戴建武[2] 叶钢[1] 

机构地区:[1]第三军医大学新桥医院泌尿外科,重庆400037 [2]中国科学院遗传与发育研究所,北京100190

出  处:《重庆医学》2014年第25期3311-3313,3316,共4页Chongqing medicine

基  金:国家自然科学基金资助项目(31000442)

摘  要:目的组织工程技术重建膀胱后对上尿路结构及功能影响的分析和评估。方法将8只雄性比格犬随机分为两组:假手术对照组(A组)4只,胶原支架修复组(B组)4只,建立B组膀胱缺损动物模型,利用胶原支架材料修复重建膀胱。两组于修复前及修复后23周行肾功能相关生化指标测定以及肾脏超声检查,术后24周行标本大体形态观察及组织学检查。结果肾脏超声检查两组动物肾脏形态、血流信号正常。大体观察两组实验动物肾脏及输尿管无结石、新生物。两组肾功能相关生化指标比较,差异无统计学意义(P>0.05)。组织学检查提示组织结构完整,两组肾单位无明显病理改变。结论利用胶原支架材料重建膀胱后对上尿路结构及功能未产生不良影响,这种组织工程方法具有较好的可行性。Objective To analyze and evaluate the effects of the tissue engineering bladder reconstruction on the upper urinary tract structure and function.Methods The 8 male beagles were randomly divided into the two groups:sham-operation group (group A,n=4)and the collagen scaffold repair group (group B,n=4).The bladder defect animal model was established in the group B by using the collagen scaffold materials to repair the bladder.The renal function related biochemical indicators were detec-ted and the renal Doppler ultrasonic examination was performed in each group before repair and in 23 weeks after repair.The speci-mens from the two groups were performed the gross morphology observation and the histology examination on postoperative 24 weeks.Results The renal Doppler ultrasound examination showed the normal kidney morphology and normal blood flow signal.In the general observation,no calculi and neoplasm were found in the kidney and ureter of the experimental dogs.The renal function related biochemical indicators had no statistically significant differences between the two groups(P〉0.05).The histological exami-nation indicated that the organization structure was integrity,the nephrons in each group had no obvious pathological changes.Con-clusion Using the collagen scaffold materials to reconstruct the canine bladder has no adverse influence on the upper urinary tract structure and function,this tissue engineering approach has good feasibility.

关 键 词:组织工程 胶原支架 膀胱重建 肾功能试验 输尿管 

分 类 号:R699.5[医药卫生—泌尿科学]

 

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