HPLC法同时测定复方氯已定含漱液中葡萄糖酸氯已定及甲硝唑的含量  

Simultaneous Determination of Chlorhexidine Gluconate and Metronidazole Content in Compound Gargle Solution Chlorhexidine Giuconaie with HPLC

作　　者：王建平[1] 戴博 杨云[1] 张兰欣[1] 迟平[1] 刘茵[1] 张娟[1] 惠金玲[1] 颜耀东[1] 

机构地区：[1]空军航空医学研究所附属医院,北京100089 [2]空军总医院药学部,北京100142

出　　处：《解放军药学学报》2014年第4期340-341,344,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的用HPLC法同时测定复方氯已定含漱液中葡萄糖酸氯已定和甲硝唑的含量。方法采用Agela Technologies C18(250 mm×4.6 mm,5μm)为色谱柱;以乙腈:磷酸-三乙胺缓冲液(pH为3.2,15∶85)为流动相;检测波长为280nm,流速1.0 ml·min-1。结果甲硝唑、葡萄糖酸氯已定回收率分别为97.05%、98.41%,RSD分别为0.59%、1.89%(n=9)。结论本方法快捷、简便、灵敏,可作为该制剂质量控制的有效方法。Obiective To establish an HPLC method for the determination of chlorhexidine gluconate and metronidazole in compound gargle solution chlorhexidine giuconaie. Methods The content of psoralen and isopsoralen was assayed on an Agela Technologies C18（ 250 mm ×4.6 mm,5 μm） column with a acetonitrile-phosphoric acid triechylamine buffer（ pH 3. 2,15∶ 85） mobile phase. The flow rate was 1. 0 ml·min- 1at a detection wavelength of 280 nm. Results The recovery of chlorhexidine gluconate and metronidazole was 97. 05% with RSD = 0. 59%（ n = 9） and98. 41% with RSD = 1. 89%（ n = 9） respectively. Conclusion This method is sensitive,simple and precise.

关 键 词：复方氯已定含漱液 葡萄糖酸氯已定 甲硝唑 HPLC 

分 类 号：R927.2[医药卫生—药学]