载脂蛋白A1基因rs670和rs5069多态性对不同体质量指数青少年血脂的影响  

Effects of Apolipoprotein A1 Gene rs670 and rs5069 Polymorphisms on the Plasma Lipid Profiles in Healthy Adolescents with Different Body Mass Index

作　　者：宋永砚 龚仁蓉[1] 张珍[1] 李元昊[1] 樊梅[1] 欧国进[1] 方定志[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041 [2]川北医学院基础医学院生物化学教研室,四川南充637007

出　　处：《中国医学科学院学报》2014年第4期369-376,共8页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(81370375);四川省应用基础研究计划(2013JY0072)~~

摘　　要：目的探讨载脂蛋白A1基因(APOA1)rs670和rs5069多态性与不同体质量指数(BMI)健康青少年血脂水平的相关性。方法根据BMI将723名青少年分成4组,从低到高依次为1组[BMI=(17.80±0.75)kg/m2]、2组[BMI=(19.39±0.32)kg/m2]、3组[BMI=(20.68±0.43)kg/m2]和4组[BMI=(23.40±2.05)kg/m2]。测定身高、体质量、腰围、臀围、血压、心率、血脂及血糖等指标,计算BMI和腰臀比,提取基因组DNA并采用聚合酶链式反应-限制性片段长度多态性法对研究群体进行APOA1 rs670和rs5069多态性分型。结果 APOA1 rs670和rs5069多态性不同基因型青少年的身高、体质量、BMI、腰围、臀围、腰臀比、血压、心率、血脂及血糖等指标在1、2和3组中差异均无统计学意义,4组rs670多态性A等位基因携带者的收缩压(P=0.017)和血糖水平(P=0.009)显著高于GG基因型青少年,rs5069多态性T等位基因携带者的身高(P=0.013)、体质量(P=0.011)和臀围(P=0.026)显著高于CC基因型青少年。结论在BMI较高的健康青少年中,APOA1 rs670多态性A等位基因与收缩压和血糖水平相关,降低体质量可能改善A等位基因对收缩压和血糖的增高作用。Objective To investigate the possible effects of apolipoprotein A1 gene （APOA1） rs670 and rs5069 polymorphisms on plasma lipid profiles in healthy adolescents with different body mass index （BMI）.Methods Totally 723 adolescents were divided into four groups according to their BMI：group 1 [BMI =（17.80±0.75） kg/m2],group2 [BMI=（19.39 ±0.32） kg/m2],group 3 [BMI=（20.68 ±0.43） kg/m2],and group 4 [BMI =（23.40 ± 2.05） kg/m2].Height,weight,waist circumference,hip circumference,blood pressure,heart rate,plasma lipids,and blood glucose were determined,BMI and waist to hip ratio （W/H ratio） were calculated,and genome DNA was extracted for analyzing the genotypes of the APOA1 rs670 and rs5069 polymorphisms by polymerase chain reaction-restriction fragment length polymorphism.Results No significant differences in height,weight,BMI,waist circumference,hip circumference,W/H ratio,blood pressure,heart rate,plasma lipids,and blood glucose between APOA1 rs670 or rs5069 genotypes were observed among group 1,group 2,and group 3.In group 4,A carriers of the rs670 polymorphism had significantly higher systolic blood pressure （P =0.017） and blood glucose levels （P =0.009） than the adolescents with the GG genotype.T carriers of the rs5069 polymorphism had significantly higher height （P =0.013）,weight （P =0.011）,and hip circumference （P =0.026） than the adolescents with the CC genotype.Conclusions In healthy adolescents with higher BMI,APOA1 rs670 polymorphism is associated with systolic blood pressure and blood glucose levels.The elevation of systolic blood pressure and blood glucose levels in A carriers of APOA1 rs670 polymorphism may be favorably modulated by weight loss.

关 键 词：载脂蛋白A1基因 体质量指数 血脂 多态性 

分 类 号：R713.8[医药卫生—妇产科学]