人参皂苷Rg3对结肠癌Caco-2细胞增殖和迁移的影响  

Effects of ginsenoside Rg3 on the proliferation and migration of colon cancer Caco-2 cells

作　　者：韩萍[1] 罗阔[1] 蒋青松[2] 黄兴兰[1] 吴世友[1] 姚小平[1] 杨志祥[1] 

机构地区：[1]重庆市中山医院肿瘤科,400013 [2]重庆医科大学药理教研室,400016

出　　处：《免疫学杂志》2014年第8期722-726,共5页Immunological Journal

基　　金：重庆市卫生局2012年医学科研计划项目(2012-2-198)

摘　　要：目的研究人参皂苷Rg3对结肠癌Caco-2细胞的增殖和迁移能力的影响,为该药物的临床应用提供实验依据。方法MTT法检测人参皂苷Rg3处理的Caco-2细胞增殖情况,AnnexinV-PI流式细胞术检测Rg3处理的Caco-2细胞凋亡情况,实时RT-PCR和Western blot检测Bax、Bcl-2和caspase-3基因表达情况变化,ELISA检测细胞培养上清中IL-6和VEGF质量浓度,划痕实验检测细胞迁移能力。结果人参皂苷Rg3可显著降低Caco-2细胞的增殖并诱导凋亡,Rg3处理的Caco-2细胞中Bax和caspase-3表达上调,Bcl-2表达下调;Rg3可以影响细胞培养上清中IL-6和VEGF因子的分泌,Rg3处理的Caco-2细胞迁移能力。结论人参皂苷Rg3促进细胞凋亡,抑制结肠癌Caco-2细胞增殖和迁移。This study aims to investigate the effects of ginsenoside Rg3 on the proliferation and migration of Caco-2 cells, and provide an experimental basis for clinical application of ginsenoside Rg3. Caco-2 cells were treated with ginsenoside Rg3, then MTT assay and flow eytometry were used to measure the cell proliferation and apoptosis rate, respectively. Also, real time RT-PCR and Western blot were applied to detect change of the mRNA and protein levels of Bax, Bcl-2 and caspase-3. The levels of IL-6 and VEGF in Caco-2 cell culture supernatants were analyzed by ELISA, and the motile ability of Caco-2 cells was detected by wound healing test. Data indicated that ginsenoside Rg3 treatment significantly decreased the proliferation but increased the apoptosis of Caco-2 cells, as compared with control. Bax and caspase-3 mRNA levels were increased in Caeo-2 cells after treatment with ginsenoside Rg3, while Bcl-2 gene expression was decreased, which were consistent with the results of Western blot analysis of protein. Furthermore, ginsenoside Rg3 treatment could reduce VEGF and IL-6 levels in Caeo-2 cells culture supernatant in a dose-dependent manner. The ginsenoside Rg3-treated Caco-2 cells also showed significant lower motile ability, compared with control. The results suggested that ginsenoside Rg3 has potent antitumor effects by inhibiting tumor growth and motile ability, as well as enhancing tumor apoptosis.

关 键 词：人参皂苷RG3 结肠癌Caco-2细胞 增殖抑制 凋亡 迁移能力 

分 类 号：R285[医药卫生—中药学]