麒麟菜多糖提取物体外抗单纯疱疹病毒1型活性及作用机制研究  

In vitro anti-herpes simplex virus type 1 activity and action mechanism of Eucheuma gelatinae polysaccharide

作　　者：金富军[1,2] 陈茂芸 马凯琦 王怀玲[1] 任哲[1] 王一飞[1] 

机构地区：[1]暨南大学生物医药研究院生物医药基地,广东广州510632 [2]暨南大学药学院,广东广州510632

出　　处：《中国卫生检验杂志》2014年第16期2288-2290,2297,共4页Chinese Journal of Health Laboratory Technology

基　　金：十二五国家科技支撑计划(SQ2011SF12B02099);国家自然科学基金面上项目(81274170);广东省渔业局重大平台项目(GD2012-D01-002)

摘　　要：目的对麒麟菜多糖的体外抗单纯疱疹病毒1型活性及抗病毒机制进行简要研究。方法采用四甲基偶氮唑蓝(MTT)法检测麒麟菜多糖的细胞毒性,空斑减数实验评价麒麟菜多糖对单纯疱疹病毒1型的抗病毒活性作用,Real-time PCR检测麒麟菜多糖对单纯疱疹病毒1型基因表达及基因组DNA复制的影响。结果实验发现麒麟菜多糖的细胞毒性非常微弱,对单纯疱疹病毒1型具有很好的抗病毒活性。Real-time PCR检测发现麒麟菜多糖能够抑制病毒早期基因和晚期基因的表达及DNA的合成。结论麒麟菜多糖对单纯疱疹病毒1型有效的杀灭作用及微弱的细胞毒性,提示其具有作为抗病毒药物开发的潜力。Objective To evaluate the in vitro anti - herpes simplex virus type 1 activity and the antiviral mechanism of Eu- cheuma gelatinae polysaccharide （EGP）. Methods The cytotoxicity of EGP was analyzed by methyl thiazoly tetrazolium （MTI＇） assay. The antiviral activities of EGP were measured by plaque reduction assay. The inhibitory effects of EGP on the ex- pression of the viral gene and cellular viral genome replication were determined by quantitative real - time PCR. Results The experimental results showed that the cytotoxicity of EGP was very weak and the EGP was found to significantly inhibit HSV - 1 infection in vitro. The qPCR results showed that EGP also inhibited the RNA synthesis of HSV - 1 early gene and late gene as well as viral DNA replication. Conclusion The weak cytotoxicity and strong anti - I4S~ - 1 activity enable EGP to be a candi- date for HSV - 1 therapy.

关 键 词：单纯疱疹病毒 麒麟菜多糖 抗病毒 

分 类 号：R978.7[医药卫生—药品]