ICP-MS直接测定脐带血中的As、Cd、Pb、Hg  

Direct determination of arsenic,cadmium,lead and mercury in cord blood by inductively coupled plasma mass spectrometry(ICP- MS)

作　　者：傅艳岚 雷永良[1] 宋瑞强[1] 纪律[1] 邹向宇[1] 

机构地区：[1]丽水市疾病预防控制中心,浙江丽水323000

出　　处：《中国卫生检验杂志》2014年第17期2467-2468,2472,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立利用电感耦合等离子体质谱仪（ICP-MS）对脐带血中As、Cd、Pb、Hg 4种元素直接测定的方法。方法采集胎儿脐带血124份,全血样品用0.05%Triton X-100、0.05%EDTA及5 mol/L氨水溶液配制的稀释剂稀释,以Li、Sc、Ge、In、Y、Tb和Bi作为在线内标,用电感耦合等离子体质谱仪（ICP-MS）直接对稀释后的脐带血中的砷、镉、铅、汞的含量进行测定分析。结果对质量控制物质（HMB59311）测定分析的结果令人满意,精密度较好,砷检出限为0.62μg/L,镉检出限为0.15μg/L,铅检出限为0.67μg/L,汞检出限为0.34μg/L,该方法回收率在95%～104%之间,精密度均〈3%。结论该方法操作简便,有较强的抗干扰能力,可以快速、准确的测定脐带血中的砷、镉、铅、汞含量,结果令人满意,可以满足分析测试的要求,适用于日常全血样品的测定。Objective To establish an ICP- MS method for direct determination of arsenic （As）, cadmium （Cd）, lead （Pb） and mercury（Hg） in cord blood. Methods A total of 124 fetal umbilical cord blood samples were collected for determination. The 0.05% Triton X - 100 -0.05% EDTA -5 mol/L ammonia solution was used to dilute the cord blood. With lithium, scan- dium, germanium, indium, yttrium, terbium and bismuth as the online internal standard, the contents of arsenic, cadmium, lead and mercury in cord blood diluent were determined by ICP - MS. Results The limits of detection were 0.62μg/L, 0.15 μg/L, 0.67 μg/L and 0.34μg/L for arsenic, cadmium, lead and mercury, respectively. The recoveries ranged from 95% to 104% and the RSDs were less than 3%. The analysis results of quality control substances turned out to be satisfactory. Conclusion This method is simple, rapid, sensitive, accurate and has high anti - interference ability. It is suitable for the routine detection of arsenic, cadmium, lead and mercury in whole blood samples.

关 键 词：电感耦合等离子体质谱仪 脐带血 元素 

分 类 号：O657.63[理学—分析化学]