南亚热带乡土树种人工纯林及混交林土壤微生物群落结构  

Soil microbial community structure of monoculture and mixed plantation stands of native tree species in south subtropical China

作　　者：罗达[1] 史作民[1] 唐敬超[1] 刘世荣[1] 卢立华[2] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京100091 [2]中国林业科学研究院热带林业实验中心,广西凭祥532600

出　　处：《应用生态学报》2014年第9期2543-2550,共8页Chinese Journal of Applied Ecology

基　　金：中国科学院碳专项(XDA05060100);林业公益性行业科研专项(201104006)资助

摘　　要：以我国南亚热带格木、马尾松人工纯林及二者混交林林地土壤为对象,运用磷脂脂肪酸(PLFAs)法研究了3种人工林土壤微生物生物量和群落结构特征.结果表明:旱季土壤微生物的PLFAs总量及各菌群的PLFAs量显著高于雨季.旱季土壤微生物的PLFAs总量、细菌PLFAs量、真菌PLFAs量、放线菌PLFAs量均为马尾松人工林最高,混交林次之,格木林最低;而雨季格木人工林土壤微生物的PLFAs总量、细菌PLFAs量、真菌PLFAs量、丛枝菌根真菌PLFAs量高于混交林,并显著高于马尾松人工林.主成分分析表明,土壤微生物群落结构组成受林分类型和季节的双重影响.冗余分析表明,土壤温湿度、pH值、全氮及铵态氮含量与特征磷脂脂肪酸之间呈显著相关关系.在全年水平上,混交林土壤真菌/细菌比值始终高于格木林和马尾松林,表明格木与马尾松混交更有利于提高土壤生态系统的稳定性.The effects of three plantation stands, Erythrophleum fordii （EF）, Pinus massoniana （PM）, and their mixed plantation （MP）, on soil microbial biomass and microbial community structure in south subtropical China were studied by the method of phospholipid fatty acids （PLFAs） analy sis. The results showed that the amounts of microbial total PLFAs and PLFAs of each microbial group in these three plantation stand soils were significantly higher in dry season than in rainy season. In dry season, the amounts of microbial total PLFAs, bacteria PLFAs, fungi PLFAs, and actinomycetes PLFAs were the highest in the PM soil, moderate in the MP soil, and the lowest in the EF soil. But in rainy season, the amounts of microbial total PLFAs, bacteria PLFAs, fungi PLFAs, and arbuscular mycorrhizal fungi （AMF） PLFAs in the EF soil were higher than in the MP soil, and were significantly higher than in the PM soil. Principal component analysis （PCA） indicated that the variations in soil microbial community structure composition were affected by both plantation types and seasons. Redundancy analysis （RDA） of soil microbial community structure and environmental factors showed that soil temperature and moisture, pH, total nitrogen content, and ammonium nitrogen content had significant correlations with PLFA signatures. In addition, the ratio of fungi PLFAs to bacteria PLFAs in the MP soil was the highest among the three stand soils within the whole year, indicating that mixed plantation stands could facilitate the stability of the soil ecosystem.

关 键 词：固氮树种 人工纯林 混交林 土壤微生物群落 磷脂脂肪酸 南亚热带 

分 类 号：S714.3[农业科学—林学]