基质金属蛋白酶7处理猪脱细胞真皮基质大鼠体内移植后组织学观察及其血管化评价  

HISTOLOGICAL OBSERVATION AND VASCULARIZATION EVALUATION OF PORCINE ACELLULAR DERMAL MATRIX PROCESSED WITH MATRIX METALLOPROTEINASE 7 AFTER IMPLANTED INTO RATS

作　　者：王芳[1] 武岩[2] 石文[1] 邵阳[1] 马印东[1] 贾军[1] 辛乃军[1] 吴秋合[1] 宋国栋[1] 

机构地区：[1]山东大学附属济南市中心医院烧伤科,济南2500131 [2]山东大学附属济南市中心医院医学影像中心,济南2500131

出　　处：《中国修复重建外科杂志》2014年第9期1161-1167,共7页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家临床重点专科建设项目经费资助项目;山东省医药卫生科技发展计划项目(2011HZ008);济南市留学人员创业计划项目(20080405);山东省科技发展计划项目(2013GSF11870)~~

摘　　要：目的探讨经基质金属蛋白酶7(matrix metalloproteinase 7,MMP-7)处理的猪脱细胞真皮基质(porcine acellular dermal matrix,P-ADM)(P-ADM-pm)植入大鼠体内后的组织学变化及其血管化过程。方法取家猪腹部皮肤采用机械法制备网织层真皮62块,随机分为A、B两组(n=31);A、B组真皮均经脱细胞处理制成P-ADM,B组再用MMP-7溶液处理制备P-ADM-pm;两组各取1块进行鉴定。取成年雄性Wistar大鼠30只,每只大鼠背部皮下左、右侧各植入1块P-ADM(A组)、P-ADM-pm(B组)。术后观察大鼠一般情况,于3、7、14、21、28 d行大体、组织学及免疫组织化学染色观察,14 d时行扫描电镜观察,比较两组移植物宿主细胞、血管浸润及组织学变化。结果术后大鼠均存活至实验结束,切口愈合良好,无明显炎性反应。大体观察示,术后7d两组移植物表面均形成一层有少量微血管的纤维膜,随时间延长微血管增多、变粗并浸润移植物,且B组较A组明显;至21 d两组纤维膜微血管减少、增粗,B组移植物完全血管化。组织学及免疫组织化学染色观察示,术后各时间点A组移植物炎性反应较B组严重;B组浅层成纤维细胞、微血管长入分别出现于术后3、7 d,并于14、21 d时贯穿移植物全层;A组分别出现于术后3、14d,至28d时均未贯穿移植物全层;各时间点B组成纤维细胞及新生微血管计数均显著多于A组(P<0.05)。14 d扫描电镜观察B组移植物内长入较多成纤维细胞并可见其分泌的胶原原纤维。结论与P-ADM比较,P-ADM-pm植入大鼠体内后,宿主对其反应与正常创伤愈合过程相似,其作为植入性支架材料具有良好的模板引导作用。Objective To investigate the histological changes and vascularization of the porcine acellular dermal matrix （P-ADM） processed with matrix metalloproteinase 7 （MMP-7） （P-ADM-pm） after implanted into rats. Methods Sixty-two pieces of porcine reticular layer dermis which were from the pig abdominal skin and obtained by using a mechanical method, were randomly divided into group A （n=31） and group B （n=31）. The porcine reticular layer dermis in 2 groups were treated with deceUularization （P-ADM）, then the P-ADM in group B were treated with processing by MMP-7 （P-ADM-pm）. Thirty adult male Wistar rats were selected. P-ADM （group A） and P-ADM-pm （group B） were subcutaneously transplanted into the left and right fascia lacuna, respectively. The implants were harvested from 6 rats at 3, 7, 14, 21, and 28 days after implantation, respectively. Gross, histochemical, and immunohistochemical observations, and scanning electron microscopy （SEM） examination were performed to observe host ceils, microvessels infiltration and histological changes in the implants. Results No rat died in the experiment, incision healed well and no obvious inflammatory reaction was seen in all rats. Gross observation suggested that the implants of 2 groups were encapsulated by a thin layer of connective tissue at 7 days after implantation. With the time of implantation, the microvessels increased and coarsened, and the changes of group B were more obvious than those of group A. At 21 days, the microvessels of 2 groups decreased, and the implants of group B showed complete vascularization. The histochemical and immunohistochemical observations showed that group A had more severe inflammatory response than group B. Fibroblasts and microvessels in group B appeared in the superficial zone of implant at 3 and 7 days after implantation and they could be observed in the center zone of implant at 14 and 21 days. However,fibroblasts and microvessels in group A appeared in the superficial zone of implant at 3 and 14 d

关 键 词：猪脱细胞真皮基质 基质金属蛋白酶7 血管化 成纤维细胞 大鼠 

分 类 号：R644[医药卫生—外科学]