二硫化碳对大鼠神经组织中轴突运输马达蛋白表达的影响  

Changes in expression of motor protein for axonal transport in nerve tissues of carbon disulfide-intoxicated rats

作　　者：王沙沙[1] 高媛[1] 王译苹 寇蕊蕊[1] 邹朝双[1] 谢克勤[1] 宋福永[1] 

机构地区：[1]山东大学公共卫生学院毒理学研究所,济南250012

出　　处：《中华劳动卫生职业病杂志》2014年第9期660-663,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金面上项目（81373043）

摘　　要：目的 研究二硫化碳染毒大鼠脊髓和坐骨神经中微管马达蛋白表达的变化,探讨二硫化碳所致周围神经病中轴突运输变化的可能分子机制.方法 成年雄性健康Wistar大鼠随机分为1个对照组和3个染毒组,每组10只.3个染毒组经口灌胃染毒二硫化碳,染毒剂量分别为200、400和600mg/kg,对照组给予相同体积的玉米油,每周6次,持续6周.染毒结束后处死动物取神经组织,用蛋白免疫印迹法（Western blot）检测脊髓和坐骨神经中dynein、dynactin和kinesin的相对含量.结果 二硫化碳染毒引起坐骨神经中dynein、dynactin和kinesin含量明显下降.与对照组比较,400和600 mg/kg组坐骨神经中dynein分别降低了23.47％和33.34％,3个染毒组坐骨神经中dynactin分别降低了19.91％、24.23％和41.30％,600 mg/kg剂量组坐骨神经中kinesin降低了25.98％,差异均有统计学意义（P＜0.01）.与对照组比较,600 mg/kg剂量组脊髓中dynactin下降了28.24％,差异有统计学意义（P＜0.01）,dynein和kinesin均无明显变化.结论 二硫化碳染毒影响了神经组织中微管马达蛋白的表达,这可能与其所致周围神经病的发生机制有关.Objective To study the changes in microtubule motor protein expression in the spinal cord and sciatic nerve of rats exposed to carbon disulfide,and to investigate the possible molecular mechanism of changes in axonal transport in carbon disulfide-induced peripheral neuropathy.Methods Healthy adult male Wistar rats were randomly divided into one control group and three experimental groups （10 rats per group）.The rats in experimental groups were intoxicated by gavage of carbon disulfide at a dose of 200,400,or 600 mg/kg 6 times a week for 6 consecutive weeks,while the rats in control group were given the same volume of corn oil by gavage.Animals were sacrificed after exposure,with nerve tissue separated.The levels of dynein,dynactin,and kinesin in the spinal cord and sciatic nerve were determined by Western blot.Results The content of dynein,dynactin,and kinesin in the sciatic nerve decreased significantly under exposure to carbon disulfide.The levels of dynein in the sciatic nerve were reduced by 23.47％ and 33.34％ at exposure doses of 400 and 600 mg/kg,respectively.The levels of dynactin in the sciatic nerve of the three experimental groups were reduced by 19.91％,24.23％,and 41.30％,respectively.The level of kinesin was reduced by 25.98％ under exposure to 600 mg/kg carbon disulfide.All the differences were statistically significant （P＜0.01）.As compared with the control group,the 600 mg/kg group experienced a 28.24％ decrease in level of dynactin in the spinal cord （P＜0.01）,but no significant change was observed in the level of dynein or kinesin.Conclusion Carbon disulfide has an impact on microtubule motor protein expression in nerve tissues,which might be involved in the development of carbon disulfide-induced peripheral neuropathy.

关 键 词：二硫化碳 神经组织 轴突运输 马达蛋白 

分 类 号：R114[医药卫生—卫生毒理学]