吉林省2009-2011年柯萨奇病毒A组16型基因亲缘关系研究  

Study on phylogenetic analysis of Coxsackievirus group A type 16 strains in Jilin province from 2009 to 2011

作　　者：周剑惠[1] 王爽[1] 屈昊[2] 魏雷雷[1] 张勇[3] 曹凤瑞[1] 程涛[1] 田鑫[1] 单元春[1] 李大强[1] 吕淑梅[1] 林琳[1] 付思美[1] 范明[1] 

机构地区：[1]吉林省疾病预防控制中心吉林省卫生厅病毒重点实验室,长春130062 [2]吉林大学临床医学院 [3]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华实验和临床病毒学杂志》2014年第4期249-251,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家科学技术艾滋病和病毒性肝炎等重大传染病防治专项传染病监测技术平台项目“辽宁及周边省市传染病病原谱流行规律研究”课题子课题“吉林省传染病病原谱流行规律研究”（编号：2012ZX10004209-003）

摘　　要：目的 分析致吉林省2009-2011年手足口病（hand,foot and mouth disease,HFMD）流行的柯萨奇病毒A组16型（Coxsackievirus group A type 16,CA16）分离株的基因亲缘关系.方法 对吉林省2009-2011年分离获得的54株CA16分离株的VP1编码区基因进行逆转录-聚合酶链反应扩增以及基因序列测定,使用MEGA 4.0软件进行基因亲缘关系分析.结果 吉林省2009-2011年54株CA16分离株有28株属于B1a基因亚型,26株属于B1b基因亚型;2009-2011年每年B1a基因亚型所占比例分别为86.7％、47.1％、31.8％,B1b基因亚型所占比例分别为13.3％、52.9％、68.2％.28株B1a基因亚型CA16形成了一个优势传播链和一个小传播链,26株B1b基因亚型CA16形成了一个优势传播链和两个小传播链.结论 B1a基因亚型所占比例呈现逐年下降趋势,B1b基因亚型所占比例呈现逐年增加趋势.吉林省2009-2011年呈现两个基因亚型各有一个优势传播链为主、各有1～2个小传播链为辅的共流行传播模式.Objective To analyze the phylogenetic characteristics of Coxsackievirus group A type 16 （CA16） causing the epidemic of hand,foot and mouth disease（HFMD） in Jilin province from 2009 to 2011.Methods To choose 54 CA16 strains isolated from HFMD in Jilin province to do the reverse transcription-polymerase chain reaction （RT-PCR） and sequences of VP1 coding genes,and to analyze the sequence by the software of Mega4.0.Results 28 strains of 54 CA16 strains were B1a genotype and 26 strains of those were B1b genotype in Jilin province from 2009 to 2011 ; the proportion of B1a genotype was 86.7%,47.1% and 31.8% yearly,and the proportion of B1b genotype was 13.3%,52.9%,68.2% yearly from 2009 to 2011.28 strains of B1a genotype formed one dominant and one small transmission chain,26 strains of B1b genotype formed one dominant and two small transmission chains.Conclusion The proportion of B1a genotype showed increased year by year,and the proportion of B1b genotype showed decreased year by year.The transmission mode was showed that the two genotypes have one dominant transmission chain and one or two small transmission chains respectively which co-circulated together.

关 键 词：柯萨奇病毒感染 基因 

分 类 号：R512.5[医药卫生—内科学]