河北省卢龙地区猪与人G9型A组轮状病毒进化关系研究  

Evolutionary relationships of group A rotavirus type G9 isolated from human and pigs in Lulong,Hebei province

作　　者：马鑫[1] 李丹地[2] 郭延青[2] 向静瑶[2] 李秀萍[3] 段招军[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]中国疾病预防控制中心病毒病预防控制所 [3]宁夏医科大学总医院

出　　处：《中华实验和临床病毒学杂志》2014年第4期252-254,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 了解河北省卢龙地区猪与人G9型A组轮状病毒（GARV）主要基因的分子特征及进化关系.方法 选取1株2008年河北卢龙地区G9型GARV阳性的腹泻仔猪粪便标本LLP48及4株2009年至2011年卢龙地区G9型GARV阳性的5岁以下住院腹泻患儿粪便标本进行主要基因片段的扩增,测序后对获得的基因序列通过MEGA、DNAStar等生物软件进行序列比对、同源性分析和系统进化分析.结果 卢龙猪GARV LLP48与4株卢龙人GARV编码的VP7、VP6、NSP2和NSP4基因具有高度同源性,核苷酸和氨基酸同源性分别是89.4％～94％和94.8％ ～98.2％;VP4片段的核苷酸（氨基酸）同源性较低,为71.4％～71.6％（68.2％～69.0％）.系统进化分析表明,LLP48编码的VP7、VP6、NSP2和NSP4基因与人来源的毒株关系密切,而VP4基因与猪来源的毒株关系密切.结论 卢龙猪GARV LLP48可能是猪的VP4与人的VP7、VP6、NSP2和NSP4自然重组.Objective To study the main genes molecular characteristics and evolutionary relationship of porcine and human group A rotavirus （GARV） type G9 in Hebei Lulong region.Methods A stool specimen from diarrhea piglet marked as LLP48 in 2008 in Hebei Lulong region,which was G9 GARV positive and 4 stool specimens collected from hospitalized children with diarrhea under the age of five from 2009 to 2011 in Lulong region which were type G9 GARV positive were selected and the main genes were amplified by RT-PCR.After sequencing,the obtained genes were analyzed through MEGA,DNAStar software and other biological sequence alignment,homology and phylogenetic analyses were performed.Results Porcine GARV LLP48 in Lulong region and four strains of Lulong human GARV were highly homologous in VP7,VP6,NSP4 and NSP2 gene fragments,and nucleotide and amino acid homology respectively was 89.4%-94% and 94.8%-98.2% ; VP4 fragment of the nucleotide homology （amino acid） is low,71.4%-71.6% （68.2%-69.0%）.Phylogenetic analysis showed that LLP48 genes encoding VP7,VP6,NSP4 and NSP2 were closely related to the strain derived from human source,and VP4 gene closely related to the strains derived from porcine source.Conclusion Lulong porcine GARV LLP48 strain may be a natural reassorted product of porcine VP4 and human VP7,VP6,NSP4 and NSP2.

关 键 词：轮状病毒属 再装配病毒 

分 类 号：R373[医药卫生—病原生物学]