千里光对小鼠体外培养胚胎的胚胎毒性研究  

Embryotoxicity of Senecionis Scandentis Hebra on in vitro cultured mouse embryos

作　　者：韩佳寅[1] 易艳[1] 梁爱华[1] 张宇实[1] 李春英[1] 赵雍[1] 崔宏玉 卢禹婷 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《药学学报》2014年第9期1267-1272,共6页Acta Pharmaceutica Sinica

基　　金：国家科技重大专项-重大新药创制(2009ZX09301-005-008);国家自然科学基金资助项目(30873434)

摘　　要：为了探讨千里光的胚胎发育毒性,采用小鼠全胚胎培养模型,将8.5天小鼠胚胎从母体分离后,在含有千里光单体成分千里光菲灵碱和克氏千里光碱的即刻离心血清中培养48 h(千里光菲灵碱的终质量浓度分别为12.5、25、50和100μg·mL-1,克氏千里光碱的终质量浓度分别为12.5、25和50μg·mL-1),观察千里光菲灵碱和克氏千里光碱对胚胎生长发育和组织器官形态分化的影响。结果表明,千里光菲灵碱和克氏千里光碱对胚胎的生长发育和形态分化均具有毒性影响,其毒性具有一定的剂量相关性。千里光所含生物碱成分对体外培养的小鼠胚胎有明显的毒性作用,提示妊娠期暴露于千里光对胎儿具有潜在的毒性。The purpose of this study is to evaluate the embryotoxicity of alkaloids in Senecionis Scandentis Hebra on in vitro cultured mouse embryos. Mouse whole embryo culture （WEC） was applied in this study. Post- implantation （8.5 d） mouse embryos were isolated from their mothers, and cultured in medium of immediately centrifuged serum （ICS） with different concentrations of seneciphylline （target concentrations were 100, 50, 25 and 12.5 μg·mL^-1） or senkirkine （target concentrations were 50, 25 and 12.5 μg·mL^-1） for 48 h. After culturing completed, the development and organic morphodifferentiation of the cultured embryos were evaluated microscopically. Treatment with seneciphylline and senkirkine had adverse effects on the development and organic morphodifferentiation of embryos. The effect also had clear dose-response. Alkaloidals in Senecionis Scandentis Hebra had embryotoxicity on cultured embryos, which indicated that pregnant people exposed to Senecionis Scandentis Hebra may get potential risk on fetus.

关 键 词：千里光 千里光菲灵碱 克氏千里光碱 千里光碱 全胚胎培养 胚胎毒性 

分 类 号：R285[医药卫生—中药学]