补中益气汤干预脾虚证模型大鼠脾脏1HNMR代谢组学机制研究  

1H NMR based metabolomics study of Bu-zhong-yi-qi-tang in the Spleen-Qi deficiency rat model

作　　者：陈磊[1,2] 向欢[3] 邢婕[1] 田俊生[1] 秦雪梅[1] 杜冠华[1,4] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]山西大学体育学院,山西太原030006 [4]中国医学科学院、北京协和医学院药物研究所,北京100050

出　　处：《药学学报》2014年第9期1320-1325,共6页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81102833,81173366,31070295)

摘　　要：应用核磁共振(1H NMR)代谢组学技术结合多元统计分析方法研究脾虚证模型大鼠脾脏中内源性代谢产物的变化规律,探讨补中益气汤治疗脾虚证的作用机制。采用大黄灌胃、负荷游泳及隔日禁食的综合造模方法复制脾虚模型,观察补中益气汤对大鼠体质量以及行为活动的影响。补中益气汤能明显改善脾虚证模型大鼠的体质量和行为活动(P<0.05、0.01)。PLS-DA结果显示补中益气汤能明显降低脾虚证模型大鼠脾脏中的乳酸、牛磺酸、次黄嘌呤的含量;升高谷氨酸、鲨肌醇的含量(P<0.01),使之趋向正常。本研究为脾虚证的发病机制及补中益气汤治疗脾虚证的作用机制研究提供理论依据。The present study aimed to investigate the effect and the mechanisms of Bu-zhong-yi-qi-tang （BZYQ） on Spleen-Qi deficiency rat＇s model using nuclear magnetic resonance （NMR） metabolomics and multivariate statistical analysis methods. The rat Spleen-Qi deficiency model was established as follows： oral administration of Radix Rhei extract, loaded swimming and starvation for 24 h. The body weight and motor behavior of the rats were measured and recorded once a week. BZYQ could significantly improve body weight and behavioral of Spleen-Qi deficiency model rats compared with the model group （P〈0.05, 0.01）. After drug administration, the changes in the levels of endogenous metabolites in the spleen including decreasing lactate, taurine and hypoxanthine, increasing glutamate and scyllo-inositol compared with the model group. The metabolomics approach is an effective tool for the investigation of the pharmacologic mechanism of BZYQ and it is helpful to further research.

关 键 词：补中益气汤 脾虚证 代谢组学 核磁共振 

分 类 号：R917[医药卫生—药物分析学]