基于色素特征荧光光谱的浮游植物分类测量方法  

Classified Measurement of Phytoplankton Based on Characteristic Fluorescence of Photosynthetic Pigments

作　　者：殷高方[1] 赵南京[1] 胡丽[1,2] 余晓娅[1] 石朝毅[1] 肖雪[1] 方丽[1] 段静波[1] 甘婷婷[1] 张玉钧[1] 刘建国[1] 刘文清[1] 

机构地区：[1]中国科学院安徽光学精密机械研究所环境光学与技术重点实验室,安徽合肥230031 [2]合肥学院建筑工程系,安徽合肥230022

出　　处：《光学学报》2014年第9期312-317,共6页Acta Optica Sinica

基　　金：国家863计划(2014AA06A509;2009AA063005);国家自然科学基金(61378041);安徽省杰出青年科学基金(1108085J19);安徽省自然科学基金(1408085MD72)

摘　　要：光合作用色素组成是浮游植物分类的重要依据。通过对蓝藻、绿藻、硅藻、甲藻和隐藻等5个门类浮游植物三维荧光光谱的差异性分析,提取了与叶绿素a、叶绿素b、叶绿素c、类胡萝卜素、藻蓝蛋白和藻红蛋白等光合色素相关的36个特征荧光光谱点,提出了基于色素特征荧光光谱的不同门类浮游植物分类测量方法。对铜绿微囊藻、小球藻、桅杆藻、光甲藻和卵形隐藻的实验结果表明:色素特征荧光光谱法对5种藻类纯种样品的测量误差分别为5.15%、5.63%、7.90%、4.85%、6.55%,对优势藻类(质量分数高于50%)的测量误差分别为7.96%、8.69%、5.44%、10.78%、15.57%,对劣势藻类(质量分数低于30%)的测量误差分别为18.29%、17.52%、20.01%、29.11%、20.14%,测量结果准确度达到了三维荧光光谱法水平,但数据量和计算时间仅是三维荧光光谱法的1.1%和2.2%,是一种快速有效的浮游植物分类测量方法。The photosynthetic pigment composition is an important basis of classification of phytoplankton. Three dimensional （3D） fluorescence spectra of five phytoplankton divisions （Cyanophyta, Chlorophyta, Bacillariophyta, Dinophyta, Cryptophyta） are analyzed and 36 characteristic fluorescence peaks which are related to chlorophyll a, chlorophyll h, chlorophyll c, carotenoid, phycocyanin and phycoerythrin are extracted. On this basis, a classified measurement method of phytoplankton based on characteristic fluorescence of photosynthetic pigments is put forward. Pure cultures and mixed cultures of Microcystis aemginosa, Chlorella vulgaris, Fragilaria sp, Glenodinium gymnodinium and Cryptomonas ovate are tested. For the five pure cultures, the measuring errors are 5. 15%, 5.63%, 7.90%, 4.85%, 6.55%. For dominant species （mass fraction higher than 50% ）, the measuring errors are 7. 96%, 8.69%, 5. 44%, 10. 78%, 15. 57%. For subordinate species （mass fraction less than 30%）, the measuring errors are 18.29 %, 17.52 %, 20.01%, 29.11%, 20.14 %. The characteristic fluorescence spectrometry is a fast and effective phytoplankton classified measurement method. Its measurement accuracy has reached the level of 3D fluorescence spectrometry, while the data volume and calculation time are only 1.1% and 2.2% of the 3Dfluorescence spectrometry.

关 键 词：光谱学 浮游植物 光合作用色素 特征荧光 分类测量 

分 类 号：X832[环境科学与工程—环境工程]