雌性压力性尿失禁的动物模型研究  

Research on a rat model of female stress urinary incontinence

作　　者：李广永[1] 吴海峰[1] 余洋[1] 吕志勇[1] 师宏斌[1] 李培军[1] 陈福宝[1] 

机构地区：[1]宁夏医科大学总医院泌尿外科,银川750000

出　　处：《中华老年医学杂志》2014年第9期1018-1020,共3页Chinese Journal of Geriatrics

基　　金：宁夏自然科学基金（NZ13164）

摘　　要：目的 探讨女性压力性尿失禁尿道组织的病理学变化和机制. 方法 雌性大鼠分娩后立即行阴道扩张4h,1周后行卵巢摘除构建压力性尿失禁大鼠模型.卵巢切除1个月后分别行清醒下和麻醉下尿流动力学检查.尿道组织取材后进一步行组织化学和Western blot检查. 结果 （1）模型组有85％的大鼠出现压力性尿失禁.（2）模型组尿道组织肌肉含量减少,同时横纹肌出现碎裂,肌纤维排列紊乱.（3）假手术组大鼠横纹肌层弹力纤维与肌纤维连接紧密,纤维连续,而尿失禁大鼠弹力纤维与肌纤维分离,出现碎裂.（4）模型组尿道组织的血管内皮生长因子含量和血管数目下降. 结论 雌性压力性尿失禁大鼠尿道的肌肉和弹力纤维出现破坏,VEGF可能是尿道组织形态学改变的重要原因.Objective To investigate the pathological changes and mechanism in the urethra by parturition-induced stress urinary incontinence.Methods Sprague Dawley female rats underwent vaginal balloon dilation for 4 hours immediately after delivery.One week later,the rats were anesthetized and both ovaries were excised.Then a rat model of stress urinary incontinence （SUI） was successfully established.One month after ovariectomy,conscious cystometry and Leak-Point Pressure （LPP） were measured by MP150.Histological examination and Western blotting were performed after functional assays.Results （1） 85％ of rats presented astress urinary incontinence in the model group.（2） The urethras in SUI rats had decreased muscles,and striated muscles showed fragmentized and disorganized.（3） Elastic fibers were long,well organized and tightly connected to the muscle bundles in sham group,while elastic fibers showed fragmentation and disorganization in the model group.（4） The protein expression of vascular endothelial growth factor （VEGF） and blood vessels were reduced in SUI rats as compared with the sham rats.Conclusions Muscle and elastic fibers in the urethra are disrupted in SUI rat.VEGF may play an important role in regulation of pathological changes in urethra.

关 键 词：压力性 尿失禁 血管内皮生长因子类 

分 类 号：R694.54[医药卫生—泌尿科学]