DNA修复基因多态性对1,3-丁二烯作业工人染色体损伤的影响  

Study on the relationship between chromosomal damage and polymorphisms of DNA repair gene among workers exposed to 1,3-butadiene

作　　者：刘楠[1] 李斌[2] 关维俊[1] 李云[1] 郑国颖[1] 程娟[2] 崔涛[2] 孟会林[2] 

机构地区：[1]河北联合大学公共卫生学院/河北省煤矿卫生与安全重点实验室,河北唐山063000 [2]中国疾病预防控制中心职业卫生与中毒控制所

出　　处：《毒理学杂志》2014年第4期278-281,共4页Journal of Toxicology

基　　金：国家自然科学基金(30471471);唐山市科技支撑计划项目(13130297z)

摘　　要：目的研究DNA修复酶基因XRCC1、XRCC2、ERCC1、ERCC2和RAD52单核苷酸多态性在1,3-丁二烯(BD)致外周血淋巴细胞染色体损伤遗传易感性中的作用。方法选取187名BD作业工人和60名行政人员作为研究对象,采用胞质分裂阻滞微核试验(CBMN)评价个体外周血淋巴细胞染色体损伤水平,聚合酶链反应-限制性片段长度多态性方法分析XRCC1、XRCC2、ERCC1、ERCC2和RAD52基因多态性。结果在校正年龄、性别、吸烟、饮酒影响因素后,BD作业工人CBMN率为7.19‰±5.43‰显著高于对照组3.18‰±2.77‰,P<0.01;使用协方差分析,发现暴露组具有XRCC1 C26304T位点CT基因型个体CBMN率显著高于CC基因型个体(8.73‰±6.20‰,6.16‰±4.92‰,P<0.05);XRCC1 G28152A位点GG基因型个体CBMN率显著高于AA基因型个体(6.97‰±5.0‰,3.69‰±3.66‰,P<0.05);ERCC1 C19007T位点TT基因型个体CBMN率显著低于CC基因型个体(3.88‰±3.58‰,7.62‰±5.63‰,P<0.05)。结论XRCC1 C26304T、G28152A和ERCC1 C19007T位点的多态性可能影响BD作业工人染色体损伤的易感性。Objective To explore the relationship between chromosomal damage induced by 1,3-butadiene（BD） and polymorphisms of DNA repair gene of XRCC1,XRCC2,ERCC1,ERCC2 and RAD52.Methods 187 BD-exposed workers and 60 controls were recruited in this study.Chromosomal damage in peripheral blood lymphocytes was measured by cytokinesis-block micronucleus（CBMN） assay.And the polymorphisms of XRCC1,XRCC2,ERCC1,ERCC2 and RAD52 were detected by PCR-RFLP.Results By comparing the CBMN of the two groups we found that the frequency in exposed workers was higher than that in control group（P 〈 0.01）.Multivariate analysis of covariance revealed that in BD-exposed workers,the XRCC1 CT genotype of C26304 T exhibited significantly higher CBMN frequency than that of CC genotypes（8.73‰ ± 6.20‰,6.16‰ ± 4.92‰,P 〈 0.05）; The XRCC1 GG genotype of G28152 A exhibited higher CBMN frequency than that of AA genotypes（6.97‰ ± 5.0‰,3.69‰ ± 3.66‰,P 〈 0.05） significantly; The ERCC1 TT genotype of C19007 T exhibited lower CBMN frequency than that of CC genotypes significantly（3.88‰ ± 3.58‰,7.62‰ ± 5.63‰,P 〈 0.05）.Conclusion The genetic polymorphisms of XRCC1 G28152 A and XRCC4 A245 G could influence the susceptibility to DNA damage in BDexposed workers.

关 键 词：1 3-丁二烯 胞质分裂阻滞微核试验 DNA损伤修复基因 基因多态性 

分 类 号：R114[医药卫生—卫生毒理学]