肺癌患者外周血单个核细胞Th1/Th2反应状态及黄芪的调节作用  

Pattern on Th1/Th2 and regulation of astragalus in lung cancer

作　　者：郑春燕[1] 肖伟[1] 

机构地区：[1]山东大学齐鲁医院呼吸科,济南250012

出　　处：《中国免疫学杂志》2002年第7期502-504,共3页Chinese Journal of Immunology

基　　金：本课题由山东省科委基金资助(981216501)

摘　　要：目的:探讨肺癌患者外周血单个核细胞（PBMC）Th1/Th2免疫反应状态,观察中药黄芪（AG）对其作用。方法:采集并分离肺癌患者和健康志愿者的PBMCs,各分为4组:其中1组即刻冻存待测,其余3组分别为不加药对照组、加 10％AG、20％AG组,培养48h,收集细胞。以IL-2、IFN-γ代表Th1型细胞因子,IL-4、IL-6、IL-10代表Th2型细胞因子,用RT-PCR方法检测肺癌患者PBMC中Th1/Th2型细胞因子mRNA的表达。结果:肺癌患者PBMC中,IL-4、IL-6和IL-10表达阳性率显著高于正常对照（P<0.05）,而IL-2无1例表达（0/23）,IFN-γ仅1例表达;经与黄芪培养后（2种浓度）,肺癌患者Th2型因子与未加AG培养组相比,IL-4、IL-6和IL-10的表达率下降（P<0.05）;而IL-2的表达率显著提高（P<0.05）。结论:肺癌患者外周血的免疫细胞呈现Th2型免疫反应优势状态;黄芪对于肺癌宿主PBMCs Th1/Th2的状态有良好的调节作用,可使肺癌患者PBMCs的Th2型优势免疫反应向Th1方向逆转。Objective: The current study investigate the immunological reaction state of Th1/Th2 cytokines expression in the peripheral blood mononuclear cells(PBMCs) from patients with lung cancer,and evaluate the effect of astragalus(AG) on it.Methods:The PBMCs from patients were divided into 4 groups:one group as pre-cultured group,and the other cells were cultured with 10%AG,20% AG and without AG (control group) ,respectively.The PBMC from 23 lung cancer patients as well as 19 healthy controls before and after the culture with AG were collected respectively. The Thl/Th2 cytokines expression were detected by RT-PCR considering IFN-r and IL-2 as Th1 cytokines and IL-4,IL-6,IL-10 as Th2 ones. Results:The results showed that the mRNA expression rate of IL-4,IL-6,IL-10 in PBMCs from lung cancer patients was obviously higher than that from the healthy controls( P < 0.05) .IL-2 expression was not detected in all patients(0.23) ,and the positive rate of IFN-r expression was 1/23; After the cultured with AG,the expression of Thl/Tli2 cytokines were changed obviously,which was refleted by a significant enhancement in IL-2 expression paralleled by a substantial decrease in IL-4,IL-6 and IL-10.Conclusion:It can be discovered that the expression of Thl cytokines was inhibited with a concomitant predominant state of Th2 cytokines in PBMCs from lung cancer patietns. AG can convert the imblant state of Th1/Th2 cytokines and make it incline to the normal state.

关 键 词：肺癌 单个核细胞 Th1/Th2反应状态 肺肿瘤 细胞活素 黄芪 逆转录-聚合酶链反应 细胞因子 

分 类 号：R273.42[医药卫生—中西医结合] R282.71[医药卫生—中医肿瘤科]