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机构地区:[1]华南农业大学亚热带农业生物资源保护与利用国家重点实验室天然农药与化学生物学教育部重点实验室,广州510642 [2]桂林医学院公共卫生学院,桂林541004
出 处:《天然产物研究与开发》2014年第9期1345-1349,1356,共6页Natural Product Research and Development
基 金:广东高校生物农药工程技术研究中心建设项目(GCZX-A1105);广西高校教育厅科研项目(2013YB164);广西自然基金项目(2014GXNSFBA118124)
摘 要:采用多种色谱技术从窿缘桉树皮中分离得到3个化合物,经1H NMR、13C NMR和质谱分析分别鉴定为3β-O-反式对羟基肉桂酰基-12-烯-28-齐墩果酸(1)、3β-O-反式对羟基肉桂酰基-2α-羟基-12-烯-28-乌苏酸(2)和3-O-methylellagic acid-4'-O-α-L-rhamnopyranoside(3)。其中,化合物1为首次从桃金娘科植物中分离得到,化合物1、2为首次从桉属植物的树皮中分离得到,化合物1~3均为首次从该植物中分离得到。分别测定分离得到的化合物对福寿螺、南方根结线虫的毒杀活性以及对斜纹夜蛾的卵巢细胞增殖抑制作用。结果表明以上3种化合物对福寿螺有较弱的毒杀活性,在50 mg/L的浓度下,72 h的死亡率分别为20.03%、22.14%和9.84%;在100 mg/L的浓度下,化合物1和2对南方根结线虫表现出较弱的毒杀活性,72 h的死亡率分别为28.12%和42.19%,化合物3没有杀线虫活性;化合物1和2对斜纹夜蛾卵巢细胞的增殖具有明显抑制作用,在20 mg/L浓度下,抑制率分别达到71.07%和84.03%,化合物3对斜纹夜蛾卵巢细胞的活性较弱,在相同测试浓度下,抑制率为44.30%。Three compounds were isolated from the bark of Eaca/xptus exserta by various column chromatographic tech- niques. Their structures were identified as 3fl-O-traru-coumareyl-12-en- 28-oleanolic acid (1), 3β-0-trans-coumaroyl- 12-en-28-ursolic acid (2) and 3-O-methylellagie acid-4'-O-σ-L- rhamnopyranoside (3) by IH NMR,13C NMR and MS analyses. Compound 1 was obtained from the family Myrtaceae for the first time, compounds 1 and 2 were isolated from the bark of Eucalyptus species for the first time,compounds 1 ~ 3 were isolated from E. exserta for the first time. All the isolated compounds were tested for their mollnscicidal activity against Pomacea canalicu^a, nematiciclal activity against Meloidogyne incognita,and growth inhibition effect against Spodoptera litura cells. The results suggested that all com- pounds possessed weak molluacicidal activity against P. canaliculata, with mortalities of 20.03% ,22. 14% and 9.84% after 72 h,respoctively,at a concentration of 50 mg/L. At a concentration of 100 mg/L,compounds 1 and 2 exhibited weak nematicidal activity against M. incogn/ta,with mortalities of 28.12% and 42.19% after 72 h,respectively. Com- pound 3 exhibited no nematieidal activity against M. incognita. Compounds 1 and 2 showed potent cytotoxic activity a- gainst $. litura cells,with inhibition rates of 71.07% and 84.03% ,respectively,at a concentration of 20 mg/L. Com- pound 3 Showed weakly activity against S. l/tufa cells,at the same concentration level,with the irfifibitory rate of 44. 30%.
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