抑制型离子色谱法测定矿泉水中的偏硅酸  

Determination of silicate in mineral waters by suppressed ion chromatography method

作　　者：李莎[1] 汪辉[1] 胡丽俐[1] 彭新凯[1,2] 夏立新[1] 徐睿鑫 

机构地区：[1]长沙市食品质量安全监督检测中心,长沙410013 [2]湖南省食品安全生产工程技术研究中心,长沙410013

出　　处：《食品科技》2014年第9期295-299,共5页Food Science and Technology

基　　金："十二五"国家科技支撑计划项目子课题(2012BAK17B17);湖南省科技计划项目(2013WK2003)

摘　　要：目的:建立一种简单、快速、准确的抑制型离子色谱方法检测矿泉水中的偏硅酸,并对方法进行确认。方法:选用高容量、强亲水性Metrosep A Supp 5阴离子分离柱,4.0 mmol/L NaAc^1.5 mmol/L NaOH淋洗液,流速为0.7 mL/min,电导检测器,样品经0.2μm滤膜过滤后直接进样。结果:在0.5~100μg/mL范围内具有良好的线性关系,相关系数为0.99931,方法检出限为0.15μg/mL,相对标准偏差(RSD)为0.554%~0.689%,样品平均加标回收率为99.0%~102.2%。结论:该方法操作简单、快速、背景干扰小,灵敏度高、重现性好,可直接用于大批量矿泉水中偏硅酸的测定。Objective： A simple, rapid and accurate ion chromatography method was developed for the determination of silicate in mineral waters using the suppressed conductivity detector. Method： Chromatographic separation was performed on the Metrosep A Supp 5 anion analytical column and suppressed conductivity detector. Experimental conditions were optimized of isocratic celuent with 4.0 mmol/L sodium acetate and 1.5 mmol/L sodium hydroxide at a flow of 0.7 mL/min, injection sample volume of 100 μL. Results： The calibration curve for silicate showed a good linearity in the range of 0.5-100μg/mL with correlation coefficient of 0.99931, the detection limit was 0.15μg/mL, RSD was 0.554%-0.689%, and the average recovery was 99.0%-102.2%. Conclusion： The results showed that this method was simple, fast, sensitive, accurate, and suitable for the analysis of mineral water samples.

关 键 词：离子色谱 偏硅酸 矿泉水 

分 类 号：TS275.1[轻工技术与工程—农产品加工及贮藏工程]