中药煎液中游离态三价铁离子测定方法研究  

Study on the determination method for the free Fe^(3+) in decoction of traditional Chinese medicine

作　　者：陈巧利[1] 华瑛 王翠玲[1] 张宁[1] 孙艳妮[1] 王征[1] 刘建利[1] 

机构地区：[1]西部资源生物与现代生物技术教育部重点实验室,西北大学生命科学学院,西安710069 [2]陕西省肿瘤医院药剂科,710061

出　　处：《药物分析杂志》2014年第9期1580-1584,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：长江学者和创新团队发展计划资助(No.IRT1174);国家自然科学基金(No..20872118;30070905)资助项目

摘　　要：目的:研究测定中药水煎液中游离态三价铁离子含量的方法。方法:以铁离子对螺环罗丹明B肼衍生物开环显色反应的催化效应,使该试剂的乙醇溶液与中药煎液混合反应5 min,采用分光光度法在563 nm测定生成的显色产物。方法对铁离子具有很高的选择性和灵敏度。结果:在铁离子浓度为1.68~22.4 mg·L-1的范围内线性关系良好,重复性较高。检测下限为0.032mg·L-1,川芎和红花煎液中游离态和总铁含量分别为17.99、107.0和63.53、378.6#g·g-1。结论:该方法操作简便,不需要分离,符合方法学验证基本要求,适用于中草药煎液中游离铁离子和总铁含量的测定。Objective：To study the determination method for the free Fe3＋ in decoctions of traditional Chinese medicine. Method：The method was based on the Fe3 ＋catalyzed conversion of spirolactam（ nonfluorescence） of rhodamine B hydrazide derivative to ringopen amide（ fluorescence）. The ethanolic solution of FD1 was mixed with the decoction of TCM for 5 min,followed by spectrophotometrical measurement of the absorbance of colored ringopen amide at 563 nm. FD1 exhibited high selectivity and sensitivity over other metal ions. Results：Trace amounts of Fe3 ＋（1.68-22.4 mg·L1） were determined with good accuracy and reproducibility; the detection limit was about 0. 032 mg·L1. The free and total amounts of Fe3 ＋in decoctions of the Ligusticum chuanxiong Hort. and Carthamus tinctorius L. were 17. 99,107. 0 and 63. 53,378. 6 μg·g1,respectively.Conclusion：This method was simple and quick,calling for no separation,which was in accordance with the fundamental requirements of methodology validation,and can be used to determine the free and total amounts of Fe3 ＋in decoction of traditional Chinese medicine.

关 键 词：铁离子 中药汤剂 川芎 红花 罗丹明B衍生物 催化光度法 分光光度法 

分 类 号：R917[医药卫生—药物分析学]