薄层色谱扫描法对减肥保健食品中酚酞的定性定量分析  

Identification and determination of phenolphthalein added into the anti-obesity product and healthcare food by TLCS

作　　者：许凤[1] 李莉[1] 张宏莲[1] 王乐平[1] 

机构地区：[1]齐齐哈尔医学院,齐齐哈尔161006

出　　处：《药物分析杂志》2014年第9期1634-1640,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：齐齐哈尔市科学技术局科学技术社会发展项目(No.SF-201105)

摘　　要：目的：建立基层适宜的减肥保健食品中添加酚酞的分析方法。方法：采用薄层色谱扫描（TLCS）法。供试品用无水乙醇超声提取,以GF254铝合金硅胶板为固定相,以乙酸乙酯-石油醚（60-90℃）-甲醇（10∶6∶1）为展开剂,采用单波长吸收扫描法,λ=254 nm,狭缝宽8 mm×0.6 mm。结果：酚酞标准曲线线性范围为4.00-20.0μg（r=0.996）,平均回收率（n=6）为100.2%。用建立的TLCS法分别对减肥保健食品中共计16个品种20个批次进行酚酞的检测,11个批次检出了酚酞,含量在1.37%-16.51%。结论：所建立的方法经与高效液相色谱-质谱（HPLC-MS）方法比较,可作为减肥保健类产品添加酚酞的定性和定量分析方法。Objective：To establish a suitable analysis method for identification and determination of phenolphthalein added into antiobesity and healthcare food. Methods：Using TLCS method,the sample was extracted with anhydrous alcohol using ultrasonic extraction. The stationary phase was the GF254 aluminium alloy silica gel plate while the developing solvent was a mixture of acetic ether,petroleum ether（ 60 - 90 ℃） and methanol（ 10∶ 6∶ 1）.TLCS single wavelength absorption was used,λ = 254 nm,narrow slot：8 mm × 0. 6 mm. Results：The calibration curve was linear in the range of 4. 0020. 0 μg（ r = 0. 996） for phenolphthalein,and the average recovery（ n= 6） was 100. 2% for phenolphthalein. The established TLCS method was used to test a total of 16 varieties（ 20batches） of antiobesity and healthcare food,among which 11 healthcare products were detected with phenolphthalein,the content of which ranged 1. 37%16. 51%. Conclusion：The established method in comparison with HPLCMS can be used as the identification and determination method of phenolphthalein added into the products.

关 键 词：减肥保健食品 酚酞 薄层色谱扫描 高效液相色谱-质谱 

分 类 号：R917[医药卫生—药物分析学]