南方根结线虫侵染抗感黄瓜材料根系组织特征观察  

Histopathological observation of roots in cucumbers inoculated with Meloidogyne incognita

作　　者：刘雪娇[1] 程春燕[1] 杨树琼 钱春桃[1] 李季[1] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院/葫芦科作物遗传与种质创新实验室,江苏南京210095

出　　处：《南京农业大学学报》2014年第5期69-74,共6页Journal of Nanjing Agricultural University

基　　金：国家863计划项目(2012AA100202);国家科技支撑计划项目(2013BAD01B04-10);国家公益性行业(农业)科研专项(201403032);国家自然科学基金重点项目(31430075);江苏省科技支撑计划项目(BE2009310);江苏省农业科技自主创新基金[CX(11)1002];江苏省科技支撑计划(农业)项目(BE2012323);中央高校基本科研业务费专项

摘　　要：为探明抗性黄瓜材料受南方根结线虫侵染后根系组织病理学变化,以抗病黄瓜/酸黄瓜渐渗系ILs-10-1和感病普通栽培黄瓜‘北京截头’为试材,接种南方根结线虫后3、6、9、12、15和22 d取根系进行观察。结果表明:接种后3 d,ILs-10-1和‘北京截头’根尖均出现过敏性坏死;接种后6 d,在ILs-10-1和‘北京截头’根系上发现膨大的根结,两者根结数和根结大小差异不明显;但随着接种时间增加,ILs-10-1所形成的根结数明显少于‘北京截头’。对各时期横径最大的根结进行石蜡切片观察发现,ILs-10-1巨型细胞位于薄壁细胞层,数量少,体积小,周围大量的细胞在接种后6 d呈延伸状和空洞化;‘北京截头’巨型细胞位于维管束附近,数量多,体积大,周围部分细胞在接种后9 d开始出现空洞化,且易形成重根结。结论:过敏性坏死和细胞空洞化是黄瓜抗线虫侵染共有的防御特征,而抑制巨型细胞形成、周围细胞广泛空洞化和提早空洞化为抗病材料ILs-10-1的主要抗性特征。To ascertain the root hispathological changes of resistant cucumber after Meloidogyne incognita inoculation,resistant Cucu- mis sativus-hystrix introgression line ILs-10-1 and susceptible cucumber cultivar＇ Beijingjietou＇ were used in the experiment. Root samples were collected after M. incognita inoculation 3,6,9,12,15 and 22 d. The results showed that 3 d after inoculation,the root tips of ILs- 10- 1 and＇ Beijingjietou ＇ appeared hypersensitive reaction ; 6 d after inoculation, root knots were found both in ILs- 10- 1 and ＇ Beijlngjietou＇ , the size and number of root knots between them were not significant ; with the increase of inoculation days, the number of root knots in ILs-10-1 was significantly less than＇ Beijingjietou＇. To observe paraffin sections of the largest diameter root knots, it was found that the giant cells located in the parenchyma cell layer of ILs-10-1 and vascular of＇ Beijingjietou＇ respectively, and during the period of giant cells formation, the size and number of giant cells in ILs-10-1 were smaller than＇ Beijingjietou＇. After inoculation 6 d, a large number of cells surrounding the giant cells appeared elongate and vacant in ILs-10-1 ;however,in＇ Beijingjietou＇ , there were a part of cells surrounding the giant cells initiated vacant after 9 d with inoculation, and formed cluster of root knots easily. Conclusions ： It was indicated that plant cell hypersensitive reaction and cell vacant were the common characteristics in cucumber materials defense root knot nematode invasion, but inhibited the development of giant cell, surrounding cells were vacant widely and earlier cell vacant were the main resistant characteristics of ILs-10-1.

关 键 词：南方根结线虫 渐渗系 石蜡切片 巨型细胞 

分 类 号：S682.11[农业科学—观赏园艺]