基质金属蛋白酶对大鼠脑动脉瘤形成的影响  

Effect of exogenous matrix metalloproteinases and hypertension on the cerebral aneurysm formation in rats

作　　者：左右 袁先厚[2] 李雪松[3] 杜娟 赵庆锁 朱平安 

机构地区：[1] 深圳市第七人民医院神经外科518081 [2]武汉大学中南医院神经外科, 430071 [3] 惠州市中心人民医院神经外科 [4] 深圳市第七人民医院神经外科

出　　处：《中华实验外科杂志》2014年第9期1897-1899,共3页Chinese Journal of Experimental Surgery

基　　金：2014年深圳市科技创新委员会基础研究项目（20140479）

摘　　要：目的 观察天然基质金属蛋白酶（MMPs）在大鼠脑动脉瘤模型的作用.方法 健康成年雄性大鼠32只,随机分为高血压组和MMPs干预组（各16只）,前者手术造成肾性高血压,后者应用天然性MMP-2、MMP-9干预,分别于术后1、3、5周采集标本,观察脑动脉瘤形成,并检测MMPs血浆浓度及其基因表达水平变化.结果 病理学观察见高血压组有5只大鼠的脑底动脉环有明显动脉瘤形成,干预组结构基本正常.缸浆MMP-2、MMP-9浓度变化：高血压组在术后MMP-2、MMP-9水平逐渐升高,术后3周分别为（105.89±8.24）、（130.79±14.18） mg/L,术后5周分别为（124.34±11.33）、（151.85±16.54） mg/L;MMPs干预组在术后3周分别为（78.97±6.25）、（79.54±7.06） mg/L,术后5周分别为（84.30±7.03）、（86.29±9.27） mg/L,两组MMP-2、MMP-9浓度差异均有统计学意义（P＜0.05）.免疫组织化学及逆转录-聚合酶链反应（RT-PCR）检测见高血压组在术后3周MMP-2和MMP-9表达明显增加,持续至第5周,干预组仅呈弱阳性表达.结论 血压持续升高并激活MMPs可能是导致脑动脉瘤形成的重要因素.Objective To explore the effect of natural matrix metalloproteinases （MMPs） on the cerebral aneurvsm formation in rats.Methods Thirty-two healthy male adult rats were randomly divided into hypertension group and MMPs group （16 cases per group）,given operation for establishment of renal hypertension,and natural MMP-2 and MMP-9,respectively.Specimens were collected at 1st,3rd,and 5th week after operation,and the cerebral aneurysms were observed.The dynamic changes of MMP-2 and MMP-9 gene expression were examined.Results Aneurysms were obvious by pathological observation in the bifurcations of Wills arterial ring in 5 rats in hypertension group,but the structures were basically normal in MMPs group.Plasma concentrations of MMP-2 and MMP-9 were （105.89 ±8.24） and （130.79 ±14.18） mg/L at 3rd week,and （124.34 ± 11.33） and （151.85 ± 16.54） mg/L at 5th week in hypertension group; those were （78.97 ±6.25） and （79.54 ±7.06） mg/L at 3rd week,and （84.30 ±7.03） and （86.29 ±9.27） mg/L at 5th week in MMPs group.Between two groups there was significant difference in MMP-2 and MMP-9 concentrations at 3rd week and 5th week after operation （P 〈 0.05）.Immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） revealed that the expression levels of MMP-2 and MMP-9 were significantly increased at 3rd week up to 5th week after operation in hypertension group,but there was only weakly positive expression in MMPs group.Conclusion Sustaining hypertension with plentiful activation of MMPs was an important initating factor of cerebral aneurysm formation,and the mechanism was comparatively complex.

关 键 词：脑动脉瘤 基质金属蛋白酶 高血压 

分 类 号：R739.41[医药卫生—肿瘤]