坐骨神经慢性卡压后结缔组织生长因子在背根神经节中表达的变化  

Expression pattern of connective tissue growth factor in dorsal root ganglia during chronic nerve compression

作　　者：沈其孝 陈燕花[1] 陈江海[1] 李涛[1] 陈振兵[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院手外科,武汉430022

出　　处：《中华实验外科杂志》2014年第9期1996-1998,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81271967）;湖北省自然科学基金资助项目（2011CDB389）

摘　　要：目的 观察大鼠坐骨神经慢性卡压损伤后,结缔组织生长因子（CTGF）在背根神经节（DRG）的神经元中的表达变化.方法 随机选取40只SD大鼠,采用经典的MACKINNON坐骨神经慢性卡压损伤模型,分别在正常及术后第2、4、6周时取大鼠L4 ～6 DRG神经细胞进行体外培养,应用免疫组织化学染色和逆转录-聚合酶链反应（RT-PCR）检测SD大鼠DRG神经细胞中CTGF的表达和合成水平.结果 正常大鼠DRG神经元中有CTGF表达和合成.坐骨神经慢性卡压损伤后,DRG神经元中CTGF的表达水平在2、4、6周时分别为5.527 3±0.013 6、6.0567±0.141 5和4.533 3±0.0929;其合成水平分别为13.96±1.23、17.97±1.35和15.63±0.87.坐骨神经慢性卡压后DRG神经元中CTGF的表达和合成在2周时开始显著增加,4周时达到最高水平,6周时开始逐渐下降,但仍然显著高于正常水平.结论 CTGF参与周围神经慢性卡压损伤后DRG神经细胞的病理生理变化,可能与保护DRG的神经元有关.Objective To investigate the expression of connective tissue growth factor （CTGF） in the nerve cells of dorsal root ganglion （DRG） after chronic sciatic nerve compression.Methods The chronic sciatic nerve constriction injury was made in 40 male SD rats by Mackinnon method.At the 0 week,2nd week,4th week and 6.th week after the operation,L4-L6 DRG that connected to sciatic nerve were harvested.The neurons were separated from the L4-L6 DRG.Reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry were applied to detect the expression and synthesis of CTGF in the neurons from the DRG.Results The expression and synthesis of CTGF were detected in the nerve cells of normal DRG.After chronic compression of the sciatic nerve,the CTGF expression level was 5.527 3 ± 0.013 6,6.056 7 ± 0.141 5 and 4.533 3 ± 0.092 9,respectively,and CTGF synthesis was 13.96 ± 1.23,17.97 ± 1.35 and 15.63 ±0.87,respectively.At the 2nd week after chronic compression of the sciatic nerve,the expression and synthesis of CTGF were increased dramatically.At the 4th week,the expression and synthesis of CTGF were increased obviously and reached the peak.At the 6th week,the expression and synthesis of CTGF were decreased slowly,but higher than on the normal side.Conclusion CTGF not only participates in the pathophysiological process of the nerve cells of DRG after the chronic compression of sciatic nerve,but also plays an important role in protecting the nerve cells.

关 键 词：坐骨神经 慢性卡压 背根神经节 结缔组织生长因子 

分 类 号：R651.3[医药卫生—外科学]