RP-HPLC法测定新乡种植红豆杉树皮中的三尖杉宁碱  

Determination of Cephalomannine in Chinese Yew Planted in Xinxiang by RP-HPLC

作　　者：赵俊宏[1] 薛宝玉[1] 李文锋[1] 樊燕鸽[1] 

机构地区：[1]河南省科学院化学研究所有限公司,郑州450002

出　　处：《河南科学》2014年第8期1407-1410,共4页Henan Science

基　　金：河南省重点科技攻关项目(122102310045)

摘　　要：建立一种反向高效液相色谱法定量检测蛹虫草培养基中活性成分腺苷含量和虫草素含量的方法.色谱柱为Eclipse XDB-C18柱（4.6 mm×150 mm，5μm）；流动相：0.02 mol/L KH2PO4∶甲醇（体积比85∶15）溶液；流速：1.0 mL/min；检测波长：260 nm .腺苷的线性测量范围为0.5~100μg/mL（R=0.9999），平均回收率为105.3%，标准品RSD=0.76%（n=5），样品RSD=1.87%（n=5）；虫草素的线性测量范围为0.5~100μg/mL（R=0.9999），平均回收率为104.0%，标准品RSD=0.95%，样品RSD=1.23%.本测定方法线性范围宽、分离度好、具有良好精密度，可定量、准确分析不同蛹虫草培养基中活性成分腺苷和虫草素含量.A method was established for the quantitative determination of bioactive components,adenosine and cordycepin,in culture medium of Cordyceps militaris by reversed-phase high performance liquid chromatography. The separation was performed on Eclipse XDB-C18 column,The mobile phase was 0.02 mol/L phosphate buffer solution-methanol（volume ratio 85∶15）. The flow rate was 1.0 mL/min,and the detection wavelength was 260 nm. The linear measurement range of adenosine was 0.5~100μg/mL（R=0.999 9）;its average recovery was 105.3%;its standard RSD=0.76%（n=5）;and its sample RSD=1.87%（n=5）. The linear measurement range of cordycepin was 0.5~100 μg/mL（R=0.999 9）;its average recovery was 104.0%;its standard RSD=0.95%（n=5）;and its sample RSD=1.23%（n=5）. The method has the characteristics with a wide linear range,a good degree of separation and a good precision. It can be used for quantitative analysis of adenosine and cordycepin in different culture medium for artificial Cordyceps militaris.

关 键 词：三尖杉宁碱 反相高效液相色谱 红豆杉 

分 类 号：O657.7[理学—分析化学]