复方三黄酊薄层色谱鉴别与乙醇量测定的研究  

Study on identification of Compound Sanhuang Tincture by TLC and determination of ethanol

作　　者：丁长玲[1,2] 赵永德[2] 高华[1] 崔俊凤 

机构地区：[1]青岛大学,山东青岛266021 [2]滨州医学院附属医院,山东滨州256603 [3]滨州市食品药品检验检测中心,山东滨州256605

出　　处：《药学研究》2014年第10期585-588,共4页Journal of Pharmaceutical Research

基　　金：滨州医学院科技计划项目(No.BY2010KJ20)

摘　　要：目的：建立复方三黄酊的薄层色谱鉴别及乙醇量检查方法。方法用硅胶 G 薄层板，分别以甲苯-异丙醇-乙酸乙酯-甲醇-水（6：1.5：3：1.5：0.3）、三氯甲烷-甲醇-水（30：15：4）、苯-乙酸乙酯（19：1）为展开剂，温度26℃、相对湿度62%的条件下，鉴别3种黄连、黄柏和冰片药材，同时用 Agilent 6890N 气相色谱仪，测乙醇含量。结果供试品色谱中，在与对照品色谱或对照药材色谱相应的位置上，显相同颜色斑点，测得乙醇量为48.0%~50.0%。结论本法简单易行，可用于复方三黄酊的薄层鉴别和乙醇量测定。Objective To establish an advanced TLC identification method of Compound Sanhuang Tincture,and a method for the determination of ethanol. Methods The Rhizoma coptidis,Cortex phellodendri and Synthetic borneol were i-dentified with mobile phase of microemulsion form incacid by silica gel G thin layer plate. The consis of microem ulsion was toluene：toluene - isopropanol - ethyl acetate - methanol - H2 O = 6：1. 5：3：1. 5：0. 3,chloroform - methanol - H2 O（30：15：4）,benzene - ethyl acetate（19：1）. The temperature was 26 ℃,and the relative humidity 62% . The determination of ethanol was carried out with Agilent 6890N gas liquid chromatography. Results The spots in the chromatogram of the test solution corresponding to the position of each reference substance had the same color. The content of alcohol was 48. 0% ~ 50. 0% . Conclusion The method was simple and can he used for the thin layer identification of Compound Sanhuang Tincture and the determination of ethanol.

关 键 词：复方三黄酊 薄层色谱法 乙醇量 

分 类 号：R927.11[医药卫生—药学]