黄芩苷预处理对脂多糖诱导的小鼠急性肺损伤的保护作用  

Protective effects of pretreatment with baicalin on acute lung injury induced by lipopolysaccharide in mice

作　　者：吉其舰 燕宪亮[3] 赵宁军[3] 许铁[3] 

机构地区：[1]徐州医学院,江苏徐州急诊医学硕士研究生221002 [2]江苏省盱眙县人民医院 [3]徐州医学院急救与救援医学系徐州医学院附属医院急救中心

出　　处：《中国现代医药杂志》2014年第9期31-34,共4页Modern Medicine Journal of China

摘　　要：目的研究黄芩苷预处理对气管滴注脂多糖(LPS)诱导的小鼠急性肺损伤(ALI)的保护作用。方法将40只小鼠随机分成正常组(Con组)、模型组(Mod组)、黄芩苷低剂量预处理组(Baicalin-L组,25mg/kg)、黄芩苷高剂量预处理组(Baicalin-H组,100mg/kg)及地塞米松组(Dex组,5mg/kg),每组8只。采用气管内缓慢滴注5%的LPS溶液(5mg/kg)方法建立ALI模型。造模前4d,黄芩苷组分别向腹腔注射相应剂量的黄芩苷溶液,每天1次至造模当日;Dex组,前3d腹腔注射等容积的生理盐水,造模当日腹腔注射地塞米松溶液5mg/kg;Con组和Mod组在相同时间点,腹腔注射等容积的生理盐水。处死小鼠,留取肺泡灌洗液(BALF),测定BALF中IL-1β﹑IL-6和TNF-α的浓度;取右上肺组织制作病理切片,HE染色光镜观察。结果 HE染色光镜下Mod组肺组织出现炎症反应重。Baicalin组和Dex组肺组织病变较模型组有所改善。与Con组比,实验各组大鼠BALF中IL-1β﹑IL-6和TNF-α含量均明显增加;Mod组增幅最大,IL-1β﹑IL-6和TNF-α含量显著高于Dex组和Baicalin预处理组;Baicalin-L组显著高于Dex组和Baicalin-H组,差异均有统计学意义(P<0.01)。结论黄芩苷预处理可减少炎症介质释放,对LPS诱导的小鼠ALI有保护作用。Objective To study the effect of pretreatment with baicalin on acute lung injury induced by lipopolysaccharide （LPS） in mice. Methods 40 mice were randomly divided into control （Con） group, model （Mod） group, 2 dose of baicalin groups （baicalin-L 25mg/kg and baicalin-H 100mg/kg）,and dexamethasone group （Dex,Smg/kg）, 8 mice in each group. 4 days before making the model, solution of corresponding dose baicalin was injected into the peritoneal cavity of mice in baicalin group,once a day; 3 days before making the model, equal volume of normal saline was intraperitoneally injected,at the day of making model,the dexamethasone solution （Smg/kg） was intraperitoneally administered to mice in Dex group; at the same time, intraperitoneal injection of normal saline was instilled in Con group and Mod group. ALI model was established by intratracheal instillation of LPS method. For making model,5 percent of LPS （Smg/kg） was slowly instilled into the trachea of mice in experimental groups, equal volume of saline was instilled in the Con group. 3 hours after instillation of LPS, the mice of all groups were sacrificed, and the brochoalveolar lavage fluid （BALF） was collected. Then the concentrations of interleukin 113 （IL-1β）,inter- leukin 6 （IL-6） and tumor necrosis factory （TNF-α） in BALF was measured by ELISA. Pathological section was made,and the pathological change was observed by light microscopic. Results The pathology observation of lung tissue of mice in the Mod group with light microscope showed that there was serious inflammation. The pathological changes of lung tissue in baicalin group and Dex group were lower than those of Mod group. Compared with the Con group, the levels of the IL-1β, IL-6 and TNF-α in BALF of mice in experimental groups were significantly higher,the levels of the IL-1β,IL-6 and TNF-α in Mod group were higher than those in Dex and baicalin groups, the difference were statistically significant （P〈0.01）. Conclusion Baicalin pretreatment can dec

关 键 词：黄芩苷 肺损伤 脂多糖 小鼠 

分 类 号：R285.5[医药卫生—中药学]