鱼腥草不同部位挥发油组分分析及其抗菌活性研究  

Volatile oil components from different parts of Houttuynia cordata and their antibacterial activities

作　　者：伍贤进[1,2,3] 李胜华[1,2,3] 卢红梅[4] 孙媛媛[1,2,3] 李斌[1,2,3] 魏麟[1,2,3] 王捷[1,2,3] 

机构地区：[1]怀化学院生命科学系,怀化418008 [2]民族药用植物资源研究与利用湖南省重点实验室,怀化418008 [3]湘西药用植物与民族植物学湖南省高校重点实验室,怀化418008 [4]中南大学化学化工学院中药现代化研究中心,长沙410083

出　　处：《中国抗生素杂志》2014年第9期646-650,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金项目--鱼腥草种质资源及重要药用成分合成关键基因研究(30870230);湖南省科技计划项目--湘西地区特色珍贵中药材栽培和开发关键技术研究(2013FJ6090);植物学湖南省"十二五"重点建设学科

摘　　要：目的研究不同生长月份鱼腥草各部位挥发油含量变化,比较不同部位挥发性油组分和抗菌活性,为鱼腥草采收部位的选择提供依据和指导。方法用水蒸气蒸馏法从不同部位的新鲜鱼腥草中提取并测定挥发油含量,用GC-MS分析挥发油组分。用药敏纸片法和肉汤稀释法测定抗金黄色葡萄球菌和藤黄八叠球菌活性。结果不同月份和不同部位鱼腥草挥发油含量变化较大,根状茎在全年各月份含量均较高,地上茎、叶、花穗则只有在特定的月份才含有可检测的挥发油;各部位挥发油的抗菌活性以花穗最好,叶和根状茎次之,地上茎最差,其中与抗菌活性呈正相关的相对总含量依次为花穗>根状茎>叶>地上茎,组分数则分别为13、10、13和12个。结论鱼腥草药材品质与部位关系密切,其地上部分的最佳采收季节是花果盛期前后;全草采集时全年均要重视地下部分,在夏末地上部分开始衰老后更要以地下部分为主要采集部位。Objective To provide basis and guidance for choosing the right harvest parts of Houttuynia cordata. The volatile oil contents from different parts of H. cordata during different months were studied, and the corresponding antibacterial activities of the volatile oil components were evaluated. Methods The volatile oils were extracted by water-steam distillation and detected by GC-MS. The antibacterial activities to the Staphylococcus aureus and Sarcina lutea of the volatile oils were determined by Disk-diffusion method and broth dilution method. Results The volatile oil contents were different in different parts of H. cordata and in different months. The rhizome possessed relatively higher volatile oil contents in every month during the year, and for stem, leaves and spica, the volatile oils could only be detected in specific months. The antibacterial activities of volatile oil from different parts were in the order of spica 〉 leaves 〉 rhizome 〉 stem, and the corresponding numbers of volatile oil components correlating positively with antibacterial activities were 13, 13, 10 and 12, respectively. The total relative contents of volatile oils correlating positively with antibacterial activities were in the order of spica 〉 rhizome 〉 leaves 〉 stem. Conclusion There are close relations between the quality and the harvest parts in H. cordata, the best harvest period for the aboveground part is around the flowering and fruiting periods. The rhizome is very important to the quality of H. cordata, and should be paid attention to all year long, especially during the end of summer when the aboveground part begins to grow old.

关 键 词：鱼腥草 不同月份 挥发油 抗菌活性 采收部位 

分 类 号：R978.1[医药卫生—药品]