上海养殖场奶牛感染隐孢子虫的调查  

Investigation on Cryptosporidium infection in cattle, Shanghai

作　　者：储磊[1] 袁忠英[2] 沈玉娟[2] 尹建海[2] 曹建平[2] 赵宏[1] 王国忠[1] 

机构地区：[1]丹阳市人民医院,镇江212300 [2]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025

出　　处：《国际医学寄生虫病杂志》2014年第5期273-275,278,共4页International JOurnal of Medical Parasitic Diseases

摘　　要：目的 对上海地区养殖场奶牛的隐孢子虫感染状况进行调查分析. 方法 采集上海市奉贤区、金山区和青浦区5个养殖场的奶牛粪便,过滤沉淀,应用商品化试剂盒提取DNA,基于隐孢子虫18S rRNA基因进行巢式PCR扩增,琼脂糖凝胶电泳鉴定,对阳性PCR产物进行测序,鉴定虫种,并统计相应的感染率和构成比. 结果 共采集奶牛粪便样本204份,其中奉贤123份,金山43份和青浦38份,共发现36个隐孢子虫阳性样本,阳性率为17.65％ （36/204）;经测序鉴定,奶牛感染的主要虫种为安氏隐孢子虫（Cryptosporidium andersoni）,占94.44％ （34/36）,少量为牛隐孢子虫（Cryptosporidium bovis）,占5.56％ （2/36）. 结论 安氏隐孢子虫是该地区奶牛感染的主要隐孢子虫虫种,存在潜在的公共卫生危害.Objective To investigate the Cryptosporidium infection situation in cattle in Shanghai.Methods Cattle feces were collected from five different farms at three districts of Fengxian,Jinshan and Qingpu in Shanghai.DNA was extracted from feces after filtration and precipitation,and nested-PCR based on 18S rRNA gene and sequencing were used to genotype the Cryptosporidium positive samples,then the Cryptosporidium species was identified,and the infection rate and proportion were calculated.Results In total,two hundreds and four individual fecal samples were collected from Fengxian（123）,Jinshan（43） and Qingpu （38）,among which 36 samples were positive of Cryptosporidium,with the positive rate of 17.65％,most of them belonged to Cryptosporidium andersoni （94.44％,34/36）,while a few belonged to Cryptosporidium bovis （5.56％,2/36）.Conclusion Cryptosporidium andersoni was the major species infecting cattle in the areas investigated,it was a potential threat to public health.

关 键 词：隐孢子虫 牛 上海 基因分型 

分 类 号：R382.3[医药卫生—医学寄生虫学]